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Microbial cell wall distruption by electroporation

Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment t...

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Main Author: Muhammad Faris Hanif, Yusoff
Format: Undergraduates Project Papers
Language:English
Published: 2016
Subjects:
T Technology (General)
TA Engineering (General). Civil engineering (General)
Online Access:http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf
http://umpir.ump.edu.my/id/eprint/25654/
http://iportal.ump.edu.my/lib/item?id=chamo:101079&theme=UMP2
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spelling my.ump.umpir.256542019-08-16T07:45:55Z http://umpir.ump.edu.my/id/eprint/25654/ Microbial cell wall distruption by electroporation Muhammad Faris Hanif, Yusoff T Technology (General) TA Engineering (General). Civil engineering (General) Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment time towards the sample of yeast. The objective can be justified by two method, Colony Forming unit (CFU) and FESEM analysis. In CFU method, two experiment with 4 test was conducted. First experiment was 2 cm electrode distance with the treatment time for test 1 was 4 minutes and test 2 of 10 minutes. The second experiment, 4 cm electrode distance with treatment time for test 1 was 4 minutes and test 2 of 10 minutes. Based on the result, we can conclude that the trend show as the treatment time increase, the CFU count decreases. FESEM analysis show the breakup of the cell wall. Two image of untreated yeast cell and treated yeast cell with electroporation was compared ell. Treated yeast cell show the breakup of the cell wall. The optimum condition of electroporation was then being analyzed. From the result, we can conclude that the optimum condition for electroporation technique were determined to be 2 cm electrode distance and 4 minutes of treatment time due to some factor. As a conclusion, the objective of these experiment is achieved by the breaking of the cell wall and extraction of lipid content. 2016-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf Muhammad Faris Hanif, Yusoff (2016) Microbial cell wall distruption by electroporation. Faculty of Engineering Technology, Universiti Malaysia Pahang. http://iportal.ump.edu.my/lib/item?id=chamo:101079&theme=UMP2
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic T Technology (General)
TA Engineering (General). Civil engineering (General)
spellingShingle T Technology (General)
TA Engineering (General). Civil engineering (General)
Muhammad Faris Hanif, Yusoff
Microbial cell wall distruption by electroporation
description Electroporation technique was conducted in these project with an objective to disrupt the microbial cell wall. In these experiment, two factor are to be consider to know the best condition for electroporation method. Firstly, the effect of distance between two electrode and the effect of treatment time towards the sample of yeast. The objective can be justified by two method, Colony Forming unit (CFU) and FESEM analysis. In CFU method, two experiment with 4 test was conducted. First experiment was 2 cm electrode distance with the treatment time for test 1 was 4 minutes and test 2 of 10 minutes. The second experiment, 4 cm electrode distance with treatment time for test 1 was 4 minutes and test 2 of 10 minutes. Based on the result, we can conclude that the trend show as the treatment time increase, the CFU count decreases. FESEM analysis show the breakup of the cell wall. Two image of untreated yeast cell and treated yeast cell with electroporation was compared ell. Treated yeast cell show the breakup of the cell wall. The optimum condition of electroporation was then being analyzed. From the result, we can conclude that the optimum condition for electroporation technique were determined to be 2 cm electrode distance and 4 minutes of treatment time due to some factor. As a conclusion, the objective of these experiment is achieved by the breaking of the cell wall and extraction of lipid content.
format Undergraduates Project Papers
author Muhammad Faris Hanif, Yusoff
author_facet Muhammad Faris Hanif, Yusoff
author_sort Muhammad Faris Hanif, Yusoff
title Microbial cell wall distruption by electroporation
title_short Microbial cell wall distruption by electroporation
title_full Microbial cell wall distruption by electroporation
title_fullStr Microbial cell wall distruption by electroporation
title_full_unstemmed Microbial cell wall distruption by electroporation
title_sort microbial cell wall distruption by electroporation
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/25654/1/Microbial%20cell%20wall%20distruption.pdf
http://umpir.ump.edu.my/id/eprint/25654/
http://iportal.ump.edu.my/lib/item?id=chamo:101079&theme=UMP2
_version_ 1643670051275931648
score 13.211869

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