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Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology

Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-cente...

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Main Authors: Zatul Iffah, Mohd Arshad, Azura, Amid
Format: Conference or Workshop Item
Language:English
English
Published: 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf
http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf
http://umpir.ump.edu.my/id/eprint/25502/
http://dx.doi.org/10.14419/ijet.v7i4.30.22326
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spelling my.ump.umpir.255022019-11-21T03:24:52Z http://umpir.ump.edu.my/id/eprint/25502/ Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology Zatul Iffah, Mohd Arshad Azura, Amid TP Chemical technology Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel. 2018 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf pdf en http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf Zatul Iffah, Mohd Arshad and Azura, Amid (2018) Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology. In: The 4th International Conference On The Applications Of Science And Mathematics 2018, 13 - 14 Ogos 2018 , Tunku Tun Aminah Library UTHM, Parit Raja, Johor. pp. 377-382., 7 (4.30). http://dx.doi.org/10.14419/ijet.v7i4.30.22326
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Zatul Iffah, Mohd Arshad
Azura, Amid
Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
description Recombinant bromelain is a protease that was partially purified using aqueous two-phase system (ATPS). The process variables (pH, PEG 6000 and potassium phosphate concentration) were optimized on enzyme activity and partition coefficient using response surface methodology (RSM) based on a face-centered central composite design (FCCCD) model. The optimum conditions for purification were at 18.47% [w/w] PEG6000 and 13% [w/w] potassium phosphate, pH 7.0 with enzyme activity was obtained as 0.272±0.0036 unit m/L, and partition coefficient as 1.394±0.093. The recombinant bromelain was preferentially partitioned into the top phase and the band was reduced in contrast to crude sample on SDS-PAGE gel.
format Conference or Workshop Item
author Zatul Iffah, Mohd Arshad
Azura, Amid
author_facet Zatul Iffah, Mohd Arshad
Azura, Amid
author_sort Zatul Iffah, Mohd Arshad
title Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_short Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_full Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_fullStr Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_full_unstemmed Optimization of aqueous two-phase system (ATPS) of recombinant bromelain by response surface methodology
title_sort optimization of aqueous two-phase system (atps) of recombinant bromelain by response surface methodology
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/25502/1/122.%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf
http://umpir.ump.edu.my/id/eprint/25502/2/122.1%20Optimization%20of%20aqueous%20two-phase%20system%20%28ATPS%29.pdf
http://umpir.ump.edu.my/id/eprint/25502/
http://dx.doi.org/10.14419/ijet.v7i4.30.22326
_version_ 1651866929224220672
score 13.211869

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