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Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts

The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P...

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Main Authors: Mahmood, Mahmood H., Osama, A K, Makky, Essam A., Mohd Hasbi, Ab. Rahim, Nor Hafizah, Mat Ali, Nur Diana, Hazrudin
Format: Conference or Workshop Item
Language:English
English
Published: 2019
Subjects:
QA Mathematics
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf
http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf
http://umpir.ump.edu.my/id/eprint/25391/
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spelling my.ump.umpir.253912020-02-28T01:26:24Z http://umpir.ump.edu.my/id/eprint/25391/ Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts Mahmood, Mahmood H. Osama, A K Makky, Essam A. Mohd Hasbi, Ab. Rahim Nor Hafizah, Mat Ali Nur Diana, Hazrudin QA Mathematics T Technology (General) The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P. granatum, and M. nigra. Ultrasound extraction technique was used for extraction. The yield of plant extract was test for detecting phytochemical content. After the detection of compound, the plant extracts were screened at 3 different concentrations (600, 400 and 200 mg/mL) against five microorganisms (S. aureus, B. subtilis, P. vulgaris, E. coli, and C. albicans) using disc diffusion assay method for selecting the potential extract. The result showed that P. ganatum and M. nigra have highest antimicrobial activity against all the test organisms and selected for extract mixture and screened for antimicrobial activity at the same above mentioned concentrations. The DPPH scavenging activity of the individual plant extracts, P. granatum and O. stamineus gave the highest activities of 95.40 and 90.20%, respectively. Also gave the lowest IC50 of 120.2 and 330.0 μg/mL, respectively, which indicate good antioxidant agents. Both extracts were selected for antioxidant combined action effect at ratio of P. granatum: O. stamineus (v/v) 1:1, 1:2 and 2:1 and all of the mixtures exhibited good scavenging activities of 94.31, 95.40 and 90.20%, respectively. However, the mixture with ratio 1:1 was the best antioxidant due to the IC50 of 82.0 μg/mL obtained. It was concluded that the mixture of chosen extracts can be used as strong antioxidant properties as well as antimicrobial activity and the extract mixture reported as successful antifungal agent. 2019 Conference or Workshop Item PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf pdf en http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf Mahmood, Mahmood H. and Osama, A K and Makky, Essam A. and Mohd Hasbi, Ab. Rahim and Nor Hafizah, Mat Ali and Nur Diana, Hazrudin (2019) Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts. In: The 2nd international conference on applied biochemistry and biotechnology (ABB 2019), 21 - 24 Julai 2019 , Macau, China. pp. 1-17.. (Unpublished)
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
topic QA Mathematics
T Technology (General)
spellingShingle QA Mathematics
T Technology (General)
Mahmood, Mahmood H.
Osama, A K
Makky, Essam A.
Mohd Hasbi, Ab. Rahim
Nor Hafizah, Mat Ali
Nur Diana, Hazrudin
Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
description The medicinal plants were good source of phytochemicals and active compounds. In order to find the most potential plant extracts for the mixture purpose that could give combination effect for antimicrobial and antioxidant activities. The targeted plants are A. bilimbi, O. stamineus, E. longifolia, P. granatum, and M. nigra. Ultrasound extraction technique was used for extraction. The yield of plant extract was test for detecting phytochemical content. After the detection of compound, the plant extracts were screened at 3 different concentrations (600, 400 and 200 mg/mL) against five microorganisms (S. aureus, B. subtilis, P. vulgaris, E. coli, and C. albicans) using disc diffusion assay method for selecting the potential extract. The result showed that P. ganatum and M. nigra have highest antimicrobial activity against all the test organisms and selected for extract mixture and screened for antimicrobial activity at the same above mentioned concentrations. The DPPH scavenging activity of the individual plant extracts, P. granatum and O. stamineus gave the highest activities of 95.40 and 90.20%, respectively. Also gave the lowest IC50 of 120.2 and 330.0 μg/mL, respectively, which indicate good antioxidant agents. Both extracts were selected for antioxidant combined action effect at ratio of P. granatum: O. stamineus (v/v) 1:1, 1:2 and 2:1 and all of the mixtures exhibited good scavenging activities of 94.31, 95.40 and 90.20%, respectively. However, the mixture with ratio 1:1 was the best antioxidant due to the IC50 of 82.0 μg/mL obtained. It was concluded that the mixture of chosen extracts can be used as strong antioxidant properties as well as antimicrobial activity and the extract mixture reported as successful antifungal agent.
format Conference or Workshop Item
author Mahmood, Mahmood H.
Osama, A K
Makky, Essam A.
Mohd Hasbi, Ab. Rahim
Nor Hafizah, Mat Ali
Nur Diana, Hazrudin
author_facet Mahmood, Mahmood H.
Osama, A K
Makky, Essam A.
Mohd Hasbi, Ab. Rahim
Nor Hafizah, Mat Ali
Nur Diana, Hazrudin
author_sort Mahmood, Mahmood H.
title Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_short Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_full Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_fullStr Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_full_unstemmed Phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
title_sort phytochemical screening, antimicrobial and antioxidant efficacy of some plants and their mixture extracts
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/25391/1/6.%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf
http://umpir.ump.edu.my/id/eprint/25391/2/6.1%20Phytochemical%20screening%2C%20antimicrobial%20and%20antioxidant%20efficacy.pdf
http://umpir.ump.edu.my/id/eprint/25391/
_version_ 1662754716489089024
score 13.211869

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