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Comparative evaluation of protease extraction from leaves of syzygium polyanthum

In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloro...

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Main Authors:	Noorhafiza, Yahya, Zatul Iffah, Mohd Arshad, Siti Kholijah, Abdul Mudalip, Siti Zubaidah, Sulaiman, Rohaida, Che Man, Shalyda, Md Shaarani, Mohd Hairul, Ab. Rahim
Format:	Article
Language:	English
Published:	Gadjah Mada University 2019
Subjects:	QD Chemistry QP Physiology TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf http://umpir.ump.edu.my/id/eprint/25336/ https://doi.org/10.22146/ijc.40860 https://doi.org/10.22146/ijc.40860
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id	my.ump.umpir.25336
record_format	eprints
spelling	my.ump.umpir.253362019-10-25T03:32:28Z http://umpir.ump.edu.my/id/eprint/25336/ Comparative evaluation of protease extraction from leaves of syzygium polyanthum Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim QD Chemistry QP Physiology TP Chemical technology In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods. Gadjah Mada University 2019-05 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf Noorhafiza, Yahya and Zatul Iffah, Mohd Arshad and Siti Kholijah, Abdul Mudalip and Siti Zubaidah, Sulaiman and Rohaida, Che Man and Shalyda, Md Shaarani and Mohd Hairul, Ab. Rahim (2019) Comparative evaluation of protease extraction from leaves of syzygium polyanthum. Indonesian Journal of Chemistry, 19 (2). pp. 503-510. ISSN 1411-9420 (Print); 2460-1578 (Online) https://doi.org/10.22146/ijc.40860 https://doi.org/10.22146/ijc.40860
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English
topic	QD Chemistry QP Physiology TP Chemical technology
spellingShingle	QD Chemistry QP Physiology TP Chemical technology Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim Comparative evaluation of protease extraction from leaves of syzygium polyanthum
description	In this study, the comparison of four extraction methods for protease from the leaves of Syzygium polyanthum was evaluated. The methods include sodium phosphate buffer, 1-methyl-8-oxyquinolinium betaine (QB) solvent based extraction, hydrogenbond acceptor (HBA) solvent based extraction and Trichloroacetic-acetone (TCA) precipitation. The effectiveness of these extraction methods was measured based on the specific activity demonstrated by the crude protease extracted in this experiment. The results indicated that QB solvent-based extraction method could extract the crude protease from the leaves of S. polyanthum with the highest specific activity of 10.204 ± 0.329 U/mg compared to other extraction methods.
format	Article
author	Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim
author_facet	Noorhafiza, Yahya Zatul Iffah, Mohd Arshad Siti Kholijah, Abdul Mudalip Siti Zubaidah, Sulaiman Rohaida, Che Man Shalyda, Md Shaarani Mohd Hairul, Ab. Rahim
author_sort	Noorhafiza, Yahya
title	Comparative evaluation of protease extraction from leaves of syzygium polyanthum
title_short	Comparative evaluation of protease extraction from leaves of syzygium polyanthum
title_full	Comparative evaluation of protease extraction from leaves of syzygium polyanthum
title_fullStr	Comparative evaluation of protease extraction from leaves of syzygium polyanthum
title_full_unstemmed	Comparative evaluation of protease extraction from leaves of syzygium polyanthum
title_sort	comparative evaluation of protease extraction from leaves of syzygium polyanthum
publisher	Gadjah Mada University
publishDate	2019
url	http://umpir.ump.edu.my/id/eprint/25336/1/Comparative%20evaluation%20of%20protease%20extraction%20from%20leaves.pdf http://umpir.ump.edu.my/id/eprint/25336/ https://doi.org/10.22146/ijc.40860 https://doi.org/10.22146/ijc.40860
_version_	1648741194665033728
score	13.211869

Comparative evaluation of protease extraction from leaves of syzygium polyanthum

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