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Optimization of biopolymer production in shake flask using oil palm trunk sap medium

Polyhydroxybutyrate (PHB) is a biodegrable polymer which is an intracellular product produce by a bacteria. This research is conducted to optimize the biopolymer production in shake flask using oil palm trunk sap medium with Cupriavidus necator. It consists of four major phases starting with the inn...

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Main Author: Mazia, Awang
Format: Undergraduates Project Papers
Language:English
Published: 2010
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/2511/1/CD5675_MAZIA_BINTI_AWANG.pdf
http://umpir.ump.edu.my/id/eprint/2511/
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spelling my.ump.umpir.25112021-07-01T08:10:18Z http://umpir.ump.edu.my/id/eprint/2511/ Optimization of biopolymer production in shake flask using oil palm trunk sap medium Mazia, Awang TP Chemical technology Polyhydroxybutyrate (PHB) is a biodegrable polymer which is an intracellular product produce by a bacteria. This research is conducted to optimize the biopolymer production in shake flask using oil palm trunk sap medium with Cupriavidus necator. It consists of four major phases starting with the innoculum development process, followed by the fermentation process, the analysis of the process and the process optimization. The innoculum development process is done to produce the 50 ml of innoculum Cupriavidus necator in shake flask by using growth media and incubated in incubator shaker with set point values for agitation speed and temperature. This experiment is followed with the fermentation process involving the manipulation of the parameters agitation speed, temperature and percent volume of oil palm trunk sap. Among the experimental points, it is found that the highest yield of PHB is produced in conditions of 150 RPM agitation speed, 32oC temperature and 20% volume of oil palm trunk sap. This experiment was followed by mathematical analysis using Yates’ Method to study the main effects and interactive effects of changing the level of experimental variables. The calculation showed that percent volume of oil palm trunk sap has a significant main effect on PHB production while the significant interactive effect is the combination of agitation speed and percent volume of oil palm trunk sap. From the experiment for Method of the Path of Steepest Ascent the highest production of PHB occurred at parameter 31.1 oC for temperature, 138 RPM for agitation speed and 33.8% for percent volume of oil palm trunk sap 2010-12 Undergraduates Project Papers NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2511/1/CD5675_MAZIA_BINTI_AWANG.pdf Mazia, Awang (2010) Optimization of biopolymer production in shake flask using oil palm trunk sap medium. Faculty of Chemical & Natural Resources Engineering , Universiti Malaysia Pahang .
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Mazia, Awang
Optimization of biopolymer production in shake flask using oil palm trunk sap medium
description Polyhydroxybutyrate (PHB) is a biodegrable polymer which is an intracellular product produce by a bacteria. This research is conducted to optimize the biopolymer production in shake flask using oil palm trunk sap medium with Cupriavidus necator. It consists of four major phases starting with the innoculum development process, followed by the fermentation process, the analysis of the process and the process optimization. The innoculum development process is done to produce the 50 ml of innoculum Cupriavidus necator in shake flask by using growth media and incubated in incubator shaker with set point values for agitation speed and temperature. This experiment is followed with the fermentation process involving the manipulation of the parameters agitation speed, temperature and percent volume of oil palm trunk sap. Among the experimental points, it is found that the highest yield of PHB is produced in conditions of 150 RPM agitation speed, 32oC temperature and 20% volume of oil palm trunk sap. This experiment was followed by mathematical analysis using Yates’ Method to study the main effects and interactive effects of changing the level of experimental variables. The calculation showed that percent volume of oil palm trunk sap has a significant main effect on PHB production while the significant interactive effect is the combination of agitation speed and percent volume of oil palm trunk sap. From the experiment for Method of the Path of Steepest Ascent the highest production of PHB occurred at parameter 31.1 oC for temperature, 138 RPM for agitation speed and 33.8% for percent volume of oil palm trunk sap
format Undergraduates Project Papers
author Mazia, Awang
author_facet Mazia, Awang
author_sort Mazia, Awang
title Optimization of biopolymer production in shake flask using oil palm trunk sap medium
title_short Optimization of biopolymer production in shake flask using oil palm trunk sap medium
title_full Optimization of biopolymer production in shake flask using oil palm trunk sap medium
title_fullStr Optimization of biopolymer production in shake flask using oil palm trunk sap medium
title_full_unstemmed Optimization of biopolymer production in shake flask using oil palm trunk sap medium
title_sort optimization of biopolymer production in shake flask using oil palm trunk sap medium
publishDate 2010
url http://umpir.ump.edu.my/id/eprint/2511/1/CD5675_MAZIA_BINTI_AWANG.pdf
http://umpir.ump.edu.my/id/eprint/2511/
_version_ 1705056887414718464
score 13.211869

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