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Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine

Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respec...

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Main Authors: Pourjavaheri, Firoozeh, Ostovar Pour, Saeideh, Jones, Oliver A. H., Smooker, Peter M., Brkljača, Robert, Sherkat, Frank, Blanch, Ewan W., Gupta, Arun, Shanks, Robert A.
Format: Article
Language:English
Published: Elsevier Ltd 2019
Subjects:
QD Chemistry
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf
http://umpir.ump.edu.my/id/eprint/25071/
https://doi.org/10.1016/j.procbio.2019.04.010
https://doi.org/10.1016/j.procbio.2019.04.010
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spelling my.ump.umpir.250712019-10-24T06:41:14Z http://umpir.ump.edu.my/id/eprint/25071/ Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine Pourjavaheri, Firoozeh Ostovar Pour, Saeideh Jones, Oliver A. H. Smooker, Peter M. Brkljača, Robert Sherkat, Frank Blanch, Ewan W. Gupta, Arun Shanks, Robert A. QD Chemistry TP Chemical technology Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α-helix and ß-sheet based keratin could be extracted from CF using sodium sulfide and L-cysteine with high yields. This is the first report of CF keratin extraction using L-cysteine. Elsevier Ltd 2019-07 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf Pourjavaheri, Firoozeh and Ostovar Pour, Saeideh and Jones, Oliver A. H. and Smooker, Peter M. and Brkljača, Robert and Sherkat, Frank and Blanch, Ewan W. and Gupta, Arun and Shanks, Robert A. (2019) Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine. Process Biochemistry, 82. pp. 205-214. ISSN 1359-5113 https://doi.org/10.1016/j.procbio.2019.04.010 https://doi.org/10.1016/j.procbio.2019.04.010
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
TP Chemical technology
spellingShingle QD Chemistry
TP Chemical technology
Pourjavaheri, Firoozeh
Ostovar Pour, Saeideh
Jones, Oliver A. H.
Smooker, Peter M.
Brkljača, Robert
Sherkat, Frank
Blanch, Ewan W.
Gupta, Arun
Shanks, Robert A.
Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
description Keratin was extracted from different segments of disposable waste chicken feathers (CF) including the whole feathers, calamus/rachis (β-sheet) and barbs/barbules (α-helix), using sodium sulfide and L-cysteine. The yield of extracted keratin from sodium sulfide and L-cysteine was ˜88% and ˜66% respectively. The mass ratio of feathers to reducing agent was 1:20 and the reaction temperature was 40 °C for 6 h. Concentration of keratin extracted by each method was measured using the Bradford assay. The protein extracted from each feather section was characterised using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, vibrational spectroscopy including FTIR and Raman, nuclear magnetic resonance, and thermogravimetry. These results confirmed the keratin structures after each extraction methods. The study showed that α-helix and ß-sheet based keratin could be extracted from CF using sodium sulfide and L-cysteine with high yields. This is the first report of CF keratin extraction using L-cysteine.
format Article
author Pourjavaheri, Firoozeh
Ostovar Pour, Saeideh
Jones, Oliver A. H.
Smooker, Peter M.
Brkljača, Robert
Sherkat, Frank
Blanch, Ewan W.
Gupta, Arun
Shanks, Robert A.
author_facet Pourjavaheri, Firoozeh
Ostovar Pour, Saeideh
Jones, Oliver A. H.
Smooker, Peter M.
Brkljača, Robert
Sherkat, Frank
Blanch, Ewan W.
Gupta, Arun
Shanks, Robert A.
author_sort Pourjavaheri, Firoozeh
title Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
title_short Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
title_full Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
title_fullStr Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
title_full_unstemmed Extraction of keratin from waste chicken feathers using sodium sulfide and L-cysteine
title_sort extraction of keratin from waste chicken feathers using sodium sulfide and l-cysteine
publisher Elsevier Ltd
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/25071/1/Extraction%20of%20keratin%20from%20waste%20chicken%20feathers%20using%20.pdf
http://umpir.ump.edu.my/id/eprint/25071/
https://doi.org/10.1016/j.procbio.2019.04.010
https://doi.org/10.1016/j.procbio.2019.04.010
_version_ 1648741184349143040
score 13.211869

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