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Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation

Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by p...

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Main Authors: Norazwina, Zainol, Siti Natrah, Ismail
Format: Article
Language:English
Published: Department of Chemical Engineering, Diponegoro University 2019
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf
http://umpir.ump.edu.my/id/eprint/25057/
https://doi.org/10.9767/bcrec.14.2.3317.436-442
https://doi.org/10.9767/bcrec.14.2.3317.436-442
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spelling my.ump.umpir.250572019-10-24T06:20:46Z http://umpir.ump.edu.my/id/eprint/25057/ Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation Norazwina, Zainol Siti Natrah, Ismail TP Chemical technology Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by pectin methyl esterase (PME) enzyme was investigated at 25 °C and pH 9 over the reaction time range from 0 to 90 min. In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. Besides, nonlinear regression of Michaelis-Menten equation was calculated based on Euler’s and Runge-Kutta 4th order methods by using Solver supplement application. The result of kinetic constant was tested by comparing the experimental data with model predictions. It was found that Euler and Runge-Kutta method was successful in determining the kinetic parameter rather than Lineweaver-Burk plot. The application of the Michaelis-Menten equation describes the enzyme kinetic very well. From the kinetic analysis, it showed the good agreement between the result obtained and the predictions model in the production of methanol using PME enzyme. Department of Chemical Engineering, Diponegoro University 2019 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf Norazwina, Zainol and Siti Natrah, Ismail (2019) Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation. Bulletin of Chemical Reaction Engineering & Catalysis, 14 (2). pp. 436-442. ISSN 1978-2993 https://doi.org/10.9767/bcrec.14.2.3317.436-442 https://doi.org/10.9767/bcrec.14.2.3317.436-442
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Norazwina, Zainol
Siti Natrah, Ismail
Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
description Determination of kinetic parameters of enzymes is important in biotechnology research. It is also one of the most challenging processes in methanol production. The activity of enzyme is determined in term of initial rates at various substrate concentrations. The enzymatic hydrolysis of methanol by pectin methyl esterase (PME) enzyme was investigated at 25 °C and pH 9 over the reaction time range from 0 to 90 min. In this study, the parameters of the enzyme's kinetic, KM and Vmax were directly determined using a modified Michaelis-Menten equation by applying the Lineweaver-Burk plots. Besides, nonlinear regression of Michaelis-Menten equation was calculated based on Euler’s and Runge-Kutta 4th order methods by using Solver supplement application. The result of kinetic constant was tested by comparing the experimental data with model predictions. It was found that Euler and Runge-Kutta method was successful in determining the kinetic parameter rather than Lineweaver-Burk plot. The application of the Michaelis-Menten equation describes the enzyme kinetic very well. From the kinetic analysis, it showed the good agreement between the result obtained and the predictions model in the production of methanol using PME enzyme.
format Article
author Norazwina, Zainol
Siti Natrah, Ismail
author_facet Norazwina, Zainol
Siti Natrah, Ismail
author_sort Norazwina, Zainol
title Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_short Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_full Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_fullStr Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_full_unstemmed Evaluation of enzyme kinetic parameters to produce methanol using Michaelis-Menten equation
title_sort evaluation of enzyme kinetic parameters to produce methanol using michaelis-menten equation
publisher Department of Chemical Engineering, Diponegoro University
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/25057/1/Evaluation%20of%20enzyme%20kinetic%20parameters%20to%20produce%20methanol.pdf
http://umpir.ump.edu.my/id/eprint/25057/
https://doi.org/10.9767/bcrec.14.2.3317.436-442
https://doi.org/10.9767/bcrec.14.2.3317.436-442
_version_ 1648741182307565568
score 13.223943

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