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Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration

Bacterial cellulose is a type of biopolymer produced by Acetobacter xylinum in high purity, high water holding capacity, good mechanical strength, elasticity and high crystallinity. In this research, pineapple waste is used as the carbon sources for the synthesis of bacterial cellulose. The objectiv...

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Main Authors: Junaidi, Zakaria, Muhammad Azlan, Nazeri
Format: Conference or Workshop Item
Language:English
Published: 2012
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/2486/2/Optimization_of_Bacterial_Cellulose_Production_from.pdf
http://umpir.ump.edu.my/id/eprint/2486/
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spelling my.ump.umpir.24862016-12-15T01:23:26Z http://umpir.ump.edu.my/id/eprint/2486/ Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration Junaidi, Zakaria Muhammad Azlan, Nazeri TP Chemical technology Bacterial cellulose is a type of biopolymer produced by Acetobacter xylinum in high purity, high water holding capacity, good mechanical strength, elasticity and high crystallinity. In this research, pineapple waste is used as the carbon sources for the synthesis of bacterial cellulose. The objective of this study is to investigate the effect of temperature, pH and concentration of pineapple waste in the production of bacterial cellulose by Acetobacter xylinum. Parameters investigated are varied from 40% to 100% for the concentration, while the temperature is between 28°C to 32°C and pH of 4.5 to 8.5. Besides, this study also aims to optimize the production of bacterial cellulose from pineapple waste by using response surface methodology (RSM) based on the central composite design (CCD). The known value of the parameters is estimated earlier based on one factor at that time (OFAT). The results obtained from the OFAT showed the optimum condition is at pH 5.50, temperature 30°C and concentration of pineapple waste is 80 %, where the amount of bacterial cellulose dry weight is 3.3948g. According to the RSM result, the optimal conditions for bacterial cellulose were pH 5.15, temperature 30.51°C and concentration of pineapple waste is 83.32%. By using these optimal conditions, 3.4368g of bacterial cellulose is produced. The existence of bacterial cellulose is proven by Fourier Transform Infrared (FT-IR) Spectroscopy analysis based on the appearance of absorbance peak which are C-C bonding, C-O bonding, C-OH bonding and C-O-C bonding. In short, the data presented in this paper showed that pineapple waste has a great potential to use as the carbon source in production of bacterial cellulose 2012-07 Conference or Workshop Item PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/2486/2/Optimization_of_Bacterial_Cellulose_Production_from.pdf Junaidi, Zakaria and Muhammad Azlan, Nazeri (2012) Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration. In: 5th Engineering Conference, "Engineering Towards Change - Empowering Green Solutions" , 10-12th July 2012 , Kuching Sarawak. .
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Junaidi, Zakaria
Muhammad Azlan, Nazeri
Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
description Bacterial cellulose is a type of biopolymer produced by Acetobacter xylinum in high purity, high water holding capacity, good mechanical strength, elasticity and high crystallinity. In this research, pineapple waste is used as the carbon sources for the synthesis of bacterial cellulose. The objective of this study is to investigate the effect of temperature, pH and concentration of pineapple waste in the production of bacterial cellulose by Acetobacter xylinum. Parameters investigated are varied from 40% to 100% for the concentration, while the temperature is between 28°C to 32°C and pH of 4.5 to 8.5. Besides, this study also aims to optimize the production of bacterial cellulose from pineapple waste by using response surface methodology (RSM) based on the central composite design (CCD). The known value of the parameters is estimated earlier based on one factor at that time (OFAT). The results obtained from the OFAT showed the optimum condition is at pH 5.50, temperature 30°C and concentration of pineapple waste is 80 %, where the amount of bacterial cellulose dry weight is 3.3948g. According to the RSM result, the optimal conditions for bacterial cellulose were pH 5.15, temperature 30.51°C and concentration of pineapple waste is 83.32%. By using these optimal conditions, 3.4368g of bacterial cellulose is produced. The existence of bacterial cellulose is proven by Fourier Transform Infrared (FT-IR) Spectroscopy analysis based on the appearance of absorbance peak which are C-C bonding, C-O bonding, C-OH bonding and C-O-C bonding. In short, the data presented in this paper showed that pineapple waste has a great potential to use as the carbon source in production of bacterial cellulose
format Conference or Workshop Item
author Junaidi, Zakaria
Muhammad Azlan, Nazeri
author_facet Junaidi, Zakaria
Muhammad Azlan, Nazeri
author_sort Junaidi, Zakaria
title Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
title_short Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
title_full Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
title_fullStr Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
title_full_unstemmed Optimization of Bacterial Cellulose Production from Pineapple Waste: Effect of Temperature, pH and Concentration
title_sort optimization of bacterial cellulose production from pineapple waste: effect of temperature, ph and concentration
publishDate 2012
url http://umpir.ump.edu.my/id/eprint/2486/2/Optimization_of_Bacterial_Cellulose_Production_from.pdf
http://umpir.ump.edu.my/id/eprint/2486/
_version_ 1643664629780447232
score 13.211869

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