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Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line

Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improv...

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書誌詳細
第一著者: Wong, Shin Yie
フォーマット: Undergraduates Project Papers
言語:English
English
English
English
出版事項: 2018
主題:
T Technology (General)
オンライン･アクセス:http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf
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spelling my.ump.umpir.245352021-06-02T08:27:04Z http://umpir.ump.edu.my/id/eprint/24535/ Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line Wong, Shin Yie T Technology (General) Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential. 2018-01 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/3/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/24535/4/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20References.pdf Wong, Shin Yie (2018) Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line. Faculty of Engineering Technology, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang Al-Sultan Abdullah
building UMPSA Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang Al-Sultan Abdullah
content_source UMPSA Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
English
topic T Technology (General)
spellingShingle T Technology (General)
Wong, Shin Yie
Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
description Rapid increase in the demand for biopharmaceutical products has posed a challenge in the selection of high producing cell line that produce maximum amount of product. Various advances and efforts has been done on cell culture technology for Chinese Hamster Ovary (CHO) cell lines to achieve an improvement in the specific productivity of CHO cell lines. This study identifies the effects and relationship of various physiological characteristics of a panel of six CHO stable cell lines on the specific productivity (qp) of cell lines ranging from 2 to 50 pg/cell/day. We examined the correlation between physiological characteristics and specific productivity throughout the growth phase of batch culture. Results obtained were statistically analysed using Principal Component Analysis (PCA). Our research revealed that, overall, specific productivity is positively correlated to the total intracellular protein content of the cell lines. Meanwhile, specific productivity is negatively correlated to the cell density throughout the development of cell lines. However, this correlation was not observed at all-time points throughout the growth phase. Another significant finding from the research is that the correlation between physiological characteristics and specific productivity was the strongest during late exponential 5 of the growth phase as compared to early exponential.
format Undergraduates Project Papers
author Wong, Shin Yie
author_facet Wong, Shin Yie
author_sort Wong, Shin Yie
title Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_short Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_full Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_fullStr Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_full_unstemmed Statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
title_sort statistical analysis of factors affecting monoclonal antibody production by using principal component analysis : physiological characteristics of cell line
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/24535/1/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/24535/2/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/24535/3/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/24535/4/Statistical%20analysis%20of%20factors%20affecting%20monoclonal%20antibody%20production%20by%20using%20principal%20component%20analysis%20-%20physiological%20characteristics%20of%20cell%20line%20-%20References.pdf
http://umpir.ump.edu.my/id/eprint/24535/
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