Cover Image

Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation

Cancer management is associated with serious side-effects due to the harmful nature of radiation and chemotherapy on the body cells. These side-effects have necessitated the need for diversifying the alternative or complementary sources of cancer therapy. Natural products have been on the front line...

Full description

Saved in:
Bibliographic Details
Main Authors: Ukaegbu, Chinonso Ishmael, Rashid, Shah Samiur, Hamid, H. A., Normaiza, Z., Alara, Oluwaseun Ruth
Format: Article
Language:English
Published: Springer International Publishing 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/24083/1/Extracts%20of%20Hypsizygus%20tessellatus.pdf
http://umpir.ump.edu.my/id/eprint/24083/
https://doi.org/10.1007/s11694-018-9952-8
https://doi.org/10.1007/s11694-018-9952-8
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.ump.umpir.24083
record_format eprints
spelling my.ump.umpir.240832020-02-28T08:02:05Z http://umpir.ump.edu.my/id/eprint/24083/ Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation Ukaegbu, Chinonso Ishmael Rashid, Shah Samiur Hamid, H. A. Normaiza, Z. Alara, Oluwaseun Ruth TP Chemical technology Cancer management is associated with serious side-effects due to the harmful nature of radiation and chemotherapy on the body cells. These side-effects have necessitated the need for diversifying the alternative or complementary sources of cancer therapy. Natural products have been on the front line as alternative sources of anticancer agents and have attracted much attention in recent times. In this study, the anticancer activity of Hypsizygus tessellatus (white var.) caps (also known as Bunapi shimeji) extracted with acetone and ethyl acetate was evaluated in vitro against MDA-MB-231 and MCF-7 (breast cancer cell lines) and MCF-10a (Vero or normal breast cells). Likewise, the free radical scavenging and metal reducing activities of the extract were evaluated through in vitro chemical-based methods. Furthermore, the phytochemical compositions of the extracts were determined through LC–MS-QTOF-assisted mass spectroscopy. The results of this study indicated that acetone fraction had better radical scavenging activity against DPPH (IC50 = 0.76 mg/mL) and H2O2 (IC50 = 0.84 mg/mL) than ethyl acetate fraction against DPPH (IC50 = 1.10 mg/mL) and H2O2 (IC50 = 1.26 mg/mL) (p < 0.05). Additionally, the acetone fraction was observed to have more antiproliferative effects against MCF-7 (IC50 = 0.051–0.055 mg/mL) and MDA-MB-231 (IC50 = 0.122–0.131 mg/mL) compared to the ethyl acetate fraction against MCF-7 (IC50 = 0.075–0.096 mg/mL) and MDA-MB-231 (IC50 = 0.161–0.164 mg/mL) (p < 0.05). Both extracts generally had less effect on MCF-10a cells. Thus, these results suggested that Bunapi shimeji caps is a potential good natural source of anticancer agents. Springer International Publishing 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/24083/1/Extracts%20of%20Hypsizygus%20tessellatus.pdf Ukaegbu, Chinonso Ishmael and Rashid, Shah Samiur and Hamid, H. A. and Normaiza, Z. and Alara, Oluwaseun Ruth (2018) Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation. Journal of Food Measurement and Characterization. pp. 1-15. ISSN 2193-4126 (Print); 2193-4134 (Online) (In Press) https://doi.org/10.1007/s11694-018-9952-8 https://doi.org/10.1007/s11694-018-9952-8
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hamid, H. A.
Normaiza, Z.
Alara, Oluwaseun Ruth
Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
description Cancer management is associated with serious side-effects due to the harmful nature of radiation and chemotherapy on the body cells. These side-effects have necessitated the need for diversifying the alternative or complementary sources of cancer therapy. Natural products have been on the front line as alternative sources of anticancer agents and have attracted much attention in recent times. In this study, the anticancer activity of Hypsizygus tessellatus (white var.) caps (also known as Bunapi shimeji) extracted with acetone and ethyl acetate was evaluated in vitro against MDA-MB-231 and MCF-7 (breast cancer cell lines) and MCF-10a (Vero or normal breast cells). Likewise, the free radical scavenging and metal reducing activities of the extract were evaluated through in vitro chemical-based methods. Furthermore, the phytochemical compositions of the extracts were determined through LC–MS-QTOF-assisted mass spectroscopy. The results of this study indicated that acetone fraction had better radical scavenging activity against DPPH (IC50 = 0.76 mg/mL) and H2O2 (IC50 = 0.84 mg/mL) than ethyl acetate fraction against DPPH (IC50 = 1.10 mg/mL) and H2O2 (IC50 = 1.26 mg/mL) (p < 0.05). Additionally, the acetone fraction was observed to have more antiproliferative effects against MCF-7 (IC50 = 0.051–0.055 mg/mL) and MDA-MB-231 (IC50 = 0.122–0.131 mg/mL) compared to the ethyl acetate fraction against MCF-7 (IC50 = 0.075–0.096 mg/mL) and MDA-MB-231 (IC50 = 0.161–0.164 mg/mL) (p < 0.05). Both extracts generally had less effect on MCF-10a cells. Thus, these results suggested that Bunapi shimeji caps is a potential good natural source of anticancer agents.
format Article
author Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hamid, H. A.
Normaiza, Z.
Alara, Oluwaseun Ruth
author_facet Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hamid, H. A.
Normaiza, Z.
Alara, Oluwaseun Ruth
author_sort Ukaegbu, Chinonso Ishmael
title Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
title_short Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
title_full Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
title_fullStr Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
title_full_unstemmed Extracts of Hypsizygus tessellatus (white var.) caps inhibited MCF-7 and MDA-MB-231 cell lines proliferation
title_sort extracts of hypsizygus tessellatus (white var.) caps inhibited mcf-7 and mda-mb-231 cell lines proliferation
publisher Springer International Publishing
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/24083/1/Extracts%20of%20Hypsizygus%20tessellatus.pdf
http://umpir.ump.edu.my/id/eprint/24083/
https://doi.org/10.1007/s11694-018-9952-8
https://doi.org/10.1007/s11694-018-9952-8
_version_ 1662754712608309248
score 13.211869

Similar Items