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A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants

Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (Gau...

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Main Authors: Che Nur Mazadillina, Zahari, Marina, Mohd Sham, Sakina, Shahabudin, Mohd Hairul, Ab. Rahim, Nina Suhaity, Azmi
Format: Article
Language:English
Published: Innovare Academic Sciences Pvt Ltd 2019
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf
http://umpir.ump.edu.my/id/eprint/23246/
https://doi.org/10.22159/ajpcr.2019.v12i1.30283
https://doi.org/10.22159/ajpcr.2019.v12i1.30283
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spelling my.ump.umpir.232462023-04-03T07:28:52Z http://umpir.ump.edu.my/id/eprint/23246/ A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants Che Nur Mazadillina, Zahari Marina, Mohd Sham Sakina, Shahabudin Mohd Hairul, Ab. Rahim Nina Suhaity, Azmi Q Science (General) Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (Gaultheria procumbens, Strobilanthes crispus, Orthosiphon stamineus and Ficus deltoidea) using hot water extraction with some modifications. Ultraviolet (UV) spectrophotometry was conducted for purity test. Total sulfated GAG was determined by using Blyscan assay kit. By comparing results between the extract yields and total sulfated GAG, the plant consisting of high total sulfated GAG was chosen for further characterization. The selected plant sample was examined by microscopy and further analyzed by nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR) spectroscopy. Results: All four plants showed absorbance peaks between 214 and 232 nm in UV scan that represented negatively charged sugar. O. stamineus was found to contain the highest amount of sulfated GAG, 62.63 ± 0.01 µg/mg by Blyscan assay. Microscopical examination confirmed the identity of O. stamineus sample by comparing to the reference. Both NMR and FTIR analysis of O. stamineus crude yield showed presence of hydroxyl, sulfates, carboxylate, and amine groups, suggesting close resemblances to GAG structure. Conclusion: The results suggested that all four plants contained GAG compound. O. stamineus was found to exhibit the most abundant total sulfated GAG, and has potential to become a new plant-based source for GAG. Innovare Academic Sciences Pvt Ltd 2019-01 Article PeerReviewed pdf en cc_by_4 http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf Che Nur Mazadillina, Zahari and Marina, Mohd Sham and Sakina, Shahabudin and Mohd Hairul, Ab. Rahim and Nina Suhaity, Azmi (2019) A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants. Asian Journal of Pharmaceutical and Clinical Research, 12 (1). pp. 396-402. ISSN 0974-2441(print); 2455-3891(online) https://doi.org/10.22159/ajpcr.2019.v12i1.30283 https://doi.org/10.22159/ajpcr.2019.v12i1.30283
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Che Nur Mazadillina, Zahari
Marina, Mohd Sham
Sakina, Shahabudin
Mohd Hairul, Ab. Rahim
Nina Suhaity, Azmi
A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
description Objectives: The aims of this paper are to extract glycosaminoglycan (GAG) from four local medicinal plants and to characterize the crude extract with highest total sulfated GAG in order to reduce the dependency of using animals as major sources. Methods: Crude GAG was extracted from four plants (Gaultheria procumbens, Strobilanthes crispus, Orthosiphon stamineus and Ficus deltoidea) using hot water extraction with some modifications. Ultraviolet (UV) spectrophotometry was conducted for purity test. Total sulfated GAG was determined by using Blyscan assay kit. By comparing results between the extract yields and total sulfated GAG, the plant consisting of high total sulfated GAG was chosen for further characterization. The selected plant sample was examined by microscopy and further analyzed by nuclear magnetic resonance (NMR) and Fourier-transform infrared (FTIR) spectroscopy. Results: All four plants showed absorbance peaks between 214 and 232 nm in UV scan that represented negatively charged sugar. O. stamineus was found to contain the highest amount of sulfated GAG, 62.63 ± 0.01 µg/mg by Blyscan assay. Microscopical examination confirmed the identity of O. stamineus sample by comparing to the reference. Both NMR and FTIR analysis of O. stamineus crude yield showed presence of hydroxyl, sulfates, carboxylate, and amine groups, suggesting close resemblances to GAG structure. Conclusion: The results suggested that all four plants contained GAG compound. O. stamineus was found to exhibit the most abundant total sulfated GAG, and has potential to become a new plant-based source for GAG.
format Article
author Che Nur Mazadillina, Zahari
Marina, Mohd Sham
Sakina, Shahabudin
Mohd Hairul, Ab. Rahim
Nina Suhaity, Azmi
author_facet Che Nur Mazadillina, Zahari
Marina, Mohd Sham
Sakina, Shahabudin
Mohd Hairul, Ab. Rahim
Nina Suhaity, Azmi
author_sort Che Nur Mazadillina, Zahari
title A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_short A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_full A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_fullStr A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_full_unstemmed A rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
title_sort rapid quantitative dye-binding method of screening glycosaminoglycans presence in medicinal plants
publisher Innovare Academic Sciences Pvt Ltd
publishDate 2019
url http://umpir.ump.edu.my/id/eprint/23246/1/A%20rapid%20quantitative%20dye-binding%20method.pdf
http://umpir.ump.edu.my/id/eprint/23246/
https://doi.org/10.22159/ajpcr.2019.v12i1.30283
https://doi.org/10.22159/ajpcr.2019.v12i1.30283
_version_ 1762392386679341056
score 13.211869

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