Single-step purification of recombinant hepatitis B core antigen Y132A dimer from clarified Escherichia coli feedstock using a packed bed anion exchange chromatography
Hepatitis B core antigen with the mutation of Y132A (HBcAg-Y132A) was successfully expressed in Escherichia coli. The mutant HBcAg-Y132A forms dimers and is unable to self-assemble into virus-like particles (VLPs). Hence, it is a potential antigen used in the antibody-responsive biosensor for the de...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier Ltd.
2018
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| Subjects: | |
| Online Access: | http://umpir.ump.edu.my/id/eprint/22999/1/Single-step%20purification%20of%20recombinant%20hepatitis%20B%20core%20antigen%20Y132A%20dimer.pdf http://umpir.ump.edu.my/id/eprint/22999/ https://doi.org/10.1016/j.procbio.2018.03.003 |
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