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ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia

Aim: This study was carried out to detect the ESBL and MBL genes and plasmid profile analysis among the clinical isolates of Pseudomonas aeruginosa. Methods: For this study, 54 P. aeruginosa isolates were obtained from blood, skin, pus, respiratory, eyes, urine and spu...

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Main Authors: M. H. M., Nazmul, M. K. K., Fazlul, Rashid, Shah Samiur, Doustjalali, Saeid Reza, Farzana, Yasmin, Al-Jashamy, Karim, Munira, Bhuiyan, Sabet, Negar Shafiei
Format: Article
Language:English
Published: Lahore Medical & Dental College 2017
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/22906/1/ESBL%20and%20MBL%20genes%20detection%20and%20plasmid%20profile%20analysis%20from.pdf
http://umpir.ump.edu.my/id/eprint/22906/
http://www.pjmhsonline.com/2017/july_sep/pdf/815.pdf
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spelling my.ump.umpir.229062018-11-26T04:44:46Z http://umpir.ump.edu.my/id/eprint/22906/ ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia M. H. M., Nazmul M. K. K., Fazlul Rashid, Shah Samiur Doustjalali, Saeid Reza Farzana, Yasmin Al-Jashamy, Karim Munira, Bhuiyan Sabet, Negar Shafiei Q Science (General) Aim: This study was carried out to detect the ESBL and MBL genes and plasmid profile analysis among the clinical isolates of Pseudomonas aeruginosa. Methods: For this study, 54 P. aeruginosa isolates were obtained from blood, skin, pus, respiratory, eyes, urine and sputum of both paediatric and adult patients from Selayang Hospital, Kuala Lumpur. Double Disk Synergy Test (DDST) and E-test were used to detect both ESBL and MBL genes. Plasmids were detected using Close and Rodriguez with modification (1982) method. QIAprep Spin Kit was also used to extract Plasmid DNA and the method was followed according to manufacturer’s instructions. Results: Among all the P. aeruginosa isolates, 12.97% were ESBL positive, but none of the isolates were found to produce MBLs. Plasmids were detected in ten isolates with the POR of (18.51%). The overall sizes of the plasmid DNA ranged from the lowest 1.8 kb to the highest 14 kb. These ten isolates appear to harbor one or more plasmids with the maximum of four plasmids. Three isolates possessed single sized plasmids (3400bp-4600bp) while three isolates had four plasmids (1800bp-5800bp) and four isolates had two plasmids (220bp-14000bp). Conclusion: This study shows the prevalence of ESBL and MBL genes and also shows the presence of plasmids among the clinical isolates of P. aeruginosa. Lahore Medical & Dental College 2017 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22906/1/ESBL%20and%20MBL%20genes%20detection%20and%20plasmid%20profile%20analysis%20from.pdf M. H. M., Nazmul and M. K. K., Fazlul and Rashid, Shah Samiur and Doustjalali, Saeid Reza and Farzana, Yasmin and Al-Jashamy, Karim and Munira, Bhuiyan and Sabet, Negar Shafiei (2017) ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia. Pakistan Journal of Medical & Health Sciences, 11 (3). pp. 815-818. ISSN 1996-7195 http://www.pjmhsonline.com/2017/july_sep/pdf/815.pdf
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
M. H. M., Nazmul
M. K. K., Fazlul
Rashid, Shah Samiur
Doustjalali, Saeid Reza
Farzana, Yasmin
Al-Jashamy, Karim
Munira, Bhuiyan
Sabet, Negar Shafiei
ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
description Aim: This study was carried out to detect the ESBL and MBL genes and plasmid profile analysis among the clinical isolates of Pseudomonas aeruginosa. Methods: For this study, 54 P. aeruginosa isolates were obtained from blood, skin, pus, respiratory, eyes, urine and sputum of both paediatric and adult patients from Selayang Hospital, Kuala Lumpur. Double Disk Synergy Test (DDST) and E-test were used to detect both ESBL and MBL genes. Plasmids were detected using Close and Rodriguez with modification (1982) method. QIAprep Spin Kit was also used to extract Plasmid DNA and the method was followed according to manufacturer’s instructions. Results: Among all the P. aeruginosa isolates, 12.97% were ESBL positive, but none of the isolates were found to produce MBLs. Plasmids were detected in ten isolates with the POR of (18.51%). The overall sizes of the plasmid DNA ranged from the lowest 1.8 kb to the highest 14 kb. These ten isolates appear to harbor one or more plasmids with the maximum of four plasmids. Three isolates possessed single sized plasmids (3400bp-4600bp) while three isolates had four plasmids (1800bp-5800bp) and four isolates had two plasmids (220bp-14000bp). Conclusion: This study shows the prevalence of ESBL and MBL genes and also shows the presence of plasmids among the clinical isolates of P. aeruginosa.
format Article
author M. H. M., Nazmul
M. K. K., Fazlul
Rashid, Shah Samiur
Doustjalali, Saeid Reza
Farzana, Yasmin
Al-Jashamy, Karim
Munira, Bhuiyan
Sabet, Negar Shafiei
author_facet M. H. M., Nazmul
M. K. K., Fazlul
Rashid, Shah Samiur
Doustjalali, Saeid Reza
Farzana, Yasmin
Al-Jashamy, Karim
Munira, Bhuiyan
Sabet, Negar Shafiei
author_sort M. H. M., Nazmul
title ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
title_short ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
title_full ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
title_fullStr ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
title_full_unstemmed ESBL and MBL genes detection and plasmid profile analysis from Pseudomonas aeruginosa clinical isolates from Selayang Hospital, Malaysia
title_sort esbl and mbl genes detection and plasmid profile analysis from pseudomonas aeruginosa clinical isolates from selayang hospital, malaysia
publisher Lahore Medical & Dental College
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/22906/1/ESBL%20and%20MBL%20genes%20detection%20and%20plasmid%20profile%20analysis%20from.pdf
http://umpir.ump.edu.my/id/eprint/22906/
http://www.pjmhsonline.com/2017/july_sep/pdf/815.pdf
_version_ 1643669468171206656
score 13.211869

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