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Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme

The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of...

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Main Authors: Suhaily, Suhaimi, Rohaida, Che Man, Natassha, Jamil, Zatul Iffah, Mohd Arshad, Shalyda, Md Shaarani, Siti Zubaidah, Sulaiman, Siti Kholijah, Abdul Mudalip, Aizi Nor Mazila, Ramli
Format: Article
Language:English
Published: Penerbit Universiti Teknologi Malaysia 2018
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf
http://umpir.ump.edu.my/id/eprint/22866/
https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11707/6370
https://doi.org/10.11113/jt.v80.11707
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spelling my.ump.umpir.228662019-01-16T04:44:04Z http://umpir.ump.edu.my/id/eprint/22866/ Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme Suhaily, Suhaimi Rohaida, Che Man Natassha, Jamil Zatul Iffah, Mohd Arshad Shalyda, Md Shaarani Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Aizi Nor Mazila, Ramli TP Chemical technology The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD. Penerbit Universiti Teknologi Malaysia 2018-05-01 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf Suhaily, Suhaimi and Rohaida, Che Man and Natassha, Jamil and Zatul Iffah, Mohd Arshad and Shalyda, Md Shaarani and Siti Zubaidah, Sulaiman and Siti Kholijah, Abdul Mudalip and Aizi Nor Mazila, Ramli (2018) Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme. Jurnal Teknologi (Sciences and Engineering), 80 (3). pp. 165-171. ISSN 0127-9696 (print); 2180-3722 (online) https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11707/6370 https://doi.org/10.11113/jt.v80.11707
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Suhaily, Suhaimi
Rohaida, Che Man
Natassha, Jamil
Zatul Iffah, Mohd Arshad
Shalyda, Md Shaarani
Siti Zubaidah, Sulaiman
Siti Kholijah, Abdul Mudalip
Aizi Nor Mazila, Ramli
Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
description The production of cyclodextrin (CD) over the years has been increasing due to the numerous applications in industries such as in food, cosmetic, pharmaceutical and agricultural industries. However, cyclodextrin glucanotransferase (CGTase) which involved in the enzymatic reaction on the production of CD is unstable and easily denatured at extreme conditions resulted in low CD production. Hence, the enzyme immobilization technique is introduced to overcome these problems and subsequently increase the production of CD. In the present study, the CGTase was immobilized on hollow fiber membrane to increase the production of CD during the reaction. The effect of reaction conditions (types of starch, concentration of starch, temperature and pH) of the immobilized enzyme on the production of CD were investigated. Among the three types of starch tested, the soluble potato starch was the most suitable substrate for the production of CD with 4.13 mg/mL. In addition, by using 3% (w/v) of the soluble potato starch, the production of CD was 5.22 mg/mL . The optimal reaction temperature and pH were found to be at 40°C and pH 6 with 5.21 mg/mL and 4.62 mg/ml of CD, respectively. The immobilized enzyme exhibited a 1.3-3-fold increase in CD production compared to the free enzyme. Therefore, the hollow fiber membrane is suitable to be used as a support for enzyme immobilization with the high production of CD.
format Article
author Suhaily, Suhaimi
Rohaida, Che Man
Natassha, Jamil
Zatul Iffah, Mohd Arshad
Shalyda, Md Shaarani
Siti Zubaidah, Sulaiman
Siti Kholijah, Abdul Mudalip
Aizi Nor Mazila, Ramli
author_facet Suhaily, Suhaimi
Rohaida, Che Man
Natassha, Jamil
Zatul Iffah, Mohd Arshad
Shalyda, Md Shaarani
Siti Zubaidah, Sulaiman
Siti Kholijah, Abdul Mudalip
Aizi Nor Mazila, Ramli
author_sort Suhaily, Suhaimi
title Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
title_short Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
title_full Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
title_fullStr Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
title_full_unstemmed Effect of reaction conditions on the synthesis of cyclodextrin (CD) by using immobilized enzyme
title_sort effect of reaction conditions on the synthesis of cyclodextrin (cd) by using immobilized enzyme
publisher Penerbit Universiti Teknologi Malaysia
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/22866/1/Effect%20of%20reaction%20conditions%20on%20the%20synthesis%20of%20cyclodextrin%20%28CD%29%20by%20using%20immobilized%20enzyme.pdf
http://umpir.ump.edu.my/id/eprint/22866/
https://jurnalteknologi.utm.my/index.php/jurnalteknologi/article/view/11707/6370
https://doi.org/10.11113/jt.v80.11707
_version_ 1643669457660280832
score 13.211869

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