Factorial analysis on bacillus Sp. removal using garlic solution

Biofilm is a complex structure adhering to surfaces that are regularly in contact with water, consisting of colonies of bacteria and other microorganisms. Many different microbes can be found in biofilm such as Bacillus sp., Listeria monocytogenes, Staphylococcus sp., Escherichia coli and many more....

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Main Author: Siti Rahmah, Rahim
Format: Undergraduates Project Papers
Language:English
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English
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Published: 2016
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Online Access:http://umpir.ump.edu.my/id/eprint/22599/1/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Table%20of%20contents.pdf
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spelling my.ump.umpir.225992021-06-01T07:24:51Z http://umpir.ump.edu.my/id/eprint/22599/ Factorial analysis on bacillus Sp. removal using garlic solution Siti Rahmah, Rahim TP Chemical technology Biofilm is a complex structure adhering to surfaces that are regularly in contact with water, consisting of colonies of bacteria and other microorganisms. Many different microbes can be found in biofilm such as Bacillus sp., Listeria monocytogenes, Staphylococcus sp., Escherichia coli and many more. Bacillus sp. was used as biofilm model in this study. Garlic solution was used to remove Bacillus sp. In this study, garlic solution was prepared by crushing the garlic and mixed it with water. The selected factors were ratio of water to garlic (W/G) (1:1 and 1:5), ratio of garlic solution to Bacillus sp. (GS/B) (1:1 and 1:3), agitation speed (50-100 rpm) and reaction time between Bacillus sp. and garlic (12-24 hours). Design Expert software (Version 7) was used to construct experimental table where all the factors was randomized. The most contributing factor and interaction between the factors was analysed via two level factorial analysis by using the same software. The significant of this study is to find out which factors affect the most on Bacillus removal. From this study, the results showed that the most significance factors were GS/B and agitation speed, while for the interaction effect was between W/G and GS/B. As a conclusion, the Bacillus sp. removal best condition was determined at W/G at 1:1, GS/B at 1:3, agitation speed at 50 rpm and reaction time at 12 hours. The result of the best condition was 88.46% of Bacillus sp. removal. In a nutshell, the results did show the potential application of garlic solution on Bacillus sp. removal. Garlic solution can replace other commercial natural biocides especially in food industry. 2016-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22599/1/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/22599/2/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/22599/3/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/22599/4/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20References.pdf Siti Rahmah, Rahim (2016) Factorial analysis on bacillus Sp. removal using garlic solution. Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Siti Rahmah, Rahim
Factorial analysis on bacillus Sp. removal using garlic solution
description Biofilm is a complex structure adhering to surfaces that are regularly in contact with water, consisting of colonies of bacteria and other microorganisms. Many different microbes can be found in biofilm such as Bacillus sp., Listeria monocytogenes, Staphylococcus sp., Escherichia coli and many more. Bacillus sp. was used as biofilm model in this study. Garlic solution was used to remove Bacillus sp. In this study, garlic solution was prepared by crushing the garlic and mixed it with water. The selected factors were ratio of water to garlic (W/G) (1:1 and 1:5), ratio of garlic solution to Bacillus sp. (GS/B) (1:1 and 1:3), agitation speed (50-100 rpm) and reaction time between Bacillus sp. and garlic (12-24 hours). Design Expert software (Version 7) was used to construct experimental table where all the factors was randomized. The most contributing factor and interaction between the factors was analysed via two level factorial analysis by using the same software. The significant of this study is to find out which factors affect the most on Bacillus removal. From this study, the results showed that the most significance factors were GS/B and agitation speed, while for the interaction effect was between W/G and GS/B. As a conclusion, the Bacillus sp. removal best condition was determined at W/G at 1:1, GS/B at 1:3, agitation speed at 50 rpm and reaction time at 12 hours. The result of the best condition was 88.46% of Bacillus sp. removal. In a nutshell, the results did show the potential application of garlic solution on Bacillus sp. removal. Garlic solution can replace other commercial natural biocides especially in food industry.
format Undergraduates Project Papers
author Siti Rahmah, Rahim
author_facet Siti Rahmah, Rahim
author_sort Siti Rahmah, Rahim
title Factorial analysis on bacillus Sp. removal using garlic solution
title_short Factorial analysis on bacillus Sp. removal using garlic solution
title_full Factorial analysis on bacillus Sp. removal using garlic solution
title_fullStr Factorial analysis on bacillus Sp. removal using garlic solution
title_full_unstemmed Factorial analysis on bacillus Sp. removal using garlic solution
title_sort factorial analysis on bacillus sp. removal using garlic solution
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/22599/1/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/22599/2/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/22599/3/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/22599/4/Factorial%20analysis%20on%20bacillus%20Sp.%20removal%20using%20garlic%20solution%20-%20References.pdf
http://umpir.ump.edu.my/id/eprint/22599/
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