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Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents

The high death rate associated with cancer has fuelled the search for complementary cancer treatment methods in recent times. Among the leading alternative methods is natural products which have attracted much attention due to their high body tolerance. In this study, the caps of Flammulina veluti...

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Main Authors: Ukaegbu, Chinonso Ishmael, Rashid, Shah Samiur, Hazrulrizawati, Hamid, Alara, Oluwaseun Ruth
Format: Article
Language:English
Published: Elsevier 2018
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/22555/1/Acetone%20extract%20of%20Flammulina%20velutipes%20caps.pdf
http://umpir.ump.edu.my/id/eprint/22555/
https://doi.org/10.1016/j.bjbas.2018.07.012
https://doi.org/10.1016/j.bjbas.2018.07.012
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spelling my.ump.umpir.225552020-02-28T07:59:01Z http://umpir.ump.edu.my/id/eprint/22555/ Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents Ukaegbu, Chinonso Ishmael Rashid, Shah Samiur Hazrulrizawati, Hamid Alara, Oluwaseun Ruth QD Chemistry The high death rate associated with cancer has fuelled the search for complementary cancer treatment methods in recent times. Among the leading alternative methods is natural products which have attracted much attention due to their high body tolerance. In this study, the caps of Flammulina velutipes (commonly called Enoki), an edible mushroom, were extracted with different solvents and characterized for in vitro antioxidant and anticancer activities. The anticancer activity of the extract was studied against breast cancer cells (MCF-7 and MDAMD-231) and normal or Vero breast cells (MCF-10a). The radical scavenging, metal reducing power, and anticancer properties of the extracts were investigated via in vitro chemical and cell-based methods. Furthermore, a phytochemical profiling process was performed on the extract fraction with the highest level of biological activities using Liquid chromatography-mass spectrometry coupled with quadrupole time of flight (LC-MS-QTof). The results showed acetone fraction of Enoki caps to exhibit more radical scavenging activity against 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radicals (IC50=0.840 mg/mL) and hydrogen peroxide (H2O2) radicals (IC50=0.890 mg/mL) compared to the ethyl acetate fraction (IC50=0.97 mg/mL against DPPH and 1.180 mg/ mL against H2O2). Additionally, acetone fraction showed a better metal reducing property in a concentration-dependent manner. The in vitro cytotoxicity study showed a preferential level of toxicity of the acetone extract to the studied cancer cell lines compared to the Vero cells. This extract showed more cytotoxicity against MCF-7 (IC50 value ranged from 17.7 μg/mL to 38.36 μg/mL) and MDA-MB-231 (IC50 value ranged from 114.5 μg/mL to 184.2 μg/mL) compared to MCF-10a (> 250 μg/mL). These findings present F. velutipes caps as a potential natural source of antioxidant and anticancer agents. Elsevier 2018-07 Article PeerReviewed pdf en cc_by_nc_nd_4 http://umpir.ump.edu.my/id/eprint/22555/1/Acetone%20extract%20of%20Flammulina%20velutipes%20caps.pdf Ukaegbu, Chinonso Ishmael and Rashid, Shah Samiur and Hazrulrizawati, Hamid and Alara, Oluwaseun Ruth (2018) Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents. Beni-Suef University Journal of Basic and Applied Sciences, 7 (4). pp. 675-682. ISSN 2314-8535 https://doi.org/10.1016/j.bjbas.2018.07.012 https://doi.org/10.1016/j.bjbas.2018.07.012
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hazrulrizawati, Hamid
Alara, Oluwaseun Ruth
Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
description The high death rate associated with cancer has fuelled the search for complementary cancer treatment methods in recent times. Among the leading alternative methods is natural products which have attracted much attention due to their high body tolerance. In this study, the caps of Flammulina velutipes (commonly called Enoki), an edible mushroom, were extracted with different solvents and characterized for in vitro antioxidant and anticancer activities. The anticancer activity of the extract was studied against breast cancer cells (MCF-7 and MDAMD-231) and normal or Vero breast cells (MCF-10a). The radical scavenging, metal reducing power, and anticancer properties of the extracts were investigated via in vitro chemical and cell-based methods. Furthermore, a phytochemical profiling process was performed on the extract fraction with the highest level of biological activities using Liquid chromatography-mass spectrometry coupled with quadrupole time of flight (LC-MS-QTof). The results showed acetone fraction of Enoki caps to exhibit more radical scavenging activity against 2,2-diphenyl- 1-picrylhydrazyl (DPPH) free radicals (IC50=0.840 mg/mL) and hydrogen peroxide (H2O2) radicals (IC50=0.890 mg/mL) compared to the ethyl acetate fraction (IC50=0.97 mg/mL against DPPH and 1.180 mg/ mL against H2O2). Additionally, acetone fraction showed a better metal reducing property in a concentration-dependent manner. The in vitro cytotoxicity study showed a preferential level of toxicity of the acetone extract to the studied cancer cell lines compared to the Vero cells. This extract showed more cytotoxicity against MCF-7 (IC50 value ranged from 17.7 μg/mL to 38.36 μg/mL) and MDA-MB-231 (IC50 value ranged from 114.5 μg/mL to 184.2 μg/mL) compared to MCF-10a (> 250 μg/mL). These findings present F. velutipes caps as a potential natural source of antioxidant and anticancer agents.
format Article
author Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hazrulrizawati, Hamid
Alara, Oluwaseun Ruth
author_facet Ukaegbu, Chinonso Ishmael
Rashid, Shah Samiur
Hazrulrizawati, Hamid
Alara, Oluwaseun Ruth
author_sort Ukaegbu, Chinonso Ishmael
title Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
title_short Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
title_full Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
title_fullStr Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
title_full_unstemmed Acetone extract of Flammulina velutipes caps: A promising source of antioxidant and anticancer agents
title_sort acetone extract of flammulina velutipes caps: a promising source of antioxidant and anticancer agents
publisher Elsevier
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/22555/1/Acetone%20extract%20of%20Flammulina%20velutipes%20caps.pdf
http://umpir.ump.edu.my/id/eprint/22555/
https://doi.org/10.1016/j.bjbas.2018.07.012
https://doi.org/10.1016/j.bjbas.2018.07.012
_version_ 1662754709063073792
score 13.222552

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