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Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)

Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on cha...

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Main Author: Nur 'Izzaty Hazera, Nazar
Format: Undergraduates Project Papers
Language:English
English
English
English
Published: 2016
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf
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spelling my.ump.umpir.224102021-05-31T08:48:30Z http://umpir.ump.edu.my/id/eprint/22410/ Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE) Nur 'Izzaty Hazera, Nazar TP Chemical technology Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses. 2016-12 Undergraduates Project Papers NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf pdf en http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf Nur 'Izzaty Hazera, Nazar (2016) Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE). Faculty of Chemical & Natural Resources Engineering, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
English
English
English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nur 'Izzaty Hazera, Nazar
Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
description Cyclodextrin glycosyltransferase (CGTase) is an enzyme that produces cyclodextrin (CD) from starch under catalytic reaction. It is an important industrial enzyme which mostly used in food and pharmaceutical industry. The purpose of this study is to determine the effect of operating conditions on characterization and stabilization of CGTase to improve the production of cyclodextrin (CD) in the future. Therefore, the effect of starch concentration and pH on the characterization of CGTase were studied. The effect of pH and temperature on stabilization of CGTase were investigated. The best starch concentration and pH on characterization of CGTase were 3% w.v and pH 6 respectively. The most stable conditions of CGTase were detected at pH 6-8 and temperature of 40-60˚C. By using these optimum conditions, the CD production may improve in the future which in turn may be beneficial to industrial uses.
format Undergraduates Project Papers
author Nur 'Izzaty Hazera, Nazar
author_facet Nur 'Izzaty Hazera, Nazar
author_sort Nur 'Izzaty Hazera, Nazar
title Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_short Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_full Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_fullStr Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_full_unstemmed Effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (CGTASE)
title_sort effect of operating conditions on characterization and stabilization of cyclodextrin glycosyltransferase (cgtase)
publishDate 2016
url http://umpir.ump.edu.my/id/eprint/22410/1/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Table%20of%20contents.pdf
http://umpir.ump.edu.my/id/eprint/22410/2/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Abstract.pdf
http://umpir.ump.edu.my/id/eprint/22410/3/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20Chapter%201.pdf
http://umpir.ump.edu.my/id/eprint/22410/4/Effect%20of%20operating%20conditions%20on%20characterization%20and%20stabilization%20of%20cyclodextrin%20glycosyltransferase%20%28CGTASE%29%20-%20References.pdf
http://umpir.ump.edu.my/id/eprint/22410/
_version_ 1702170112304349184
score 13.211869

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