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Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro

Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a gr...

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Main Authors: Lee, Kyung Dong, Kuppusamy, Palaniselvam, Kim, Da Hye, Natanamurugaraj, Govindan, Gaanty Pragas, Maniam, Choi, Ki Choon
Format: Article
Language:English
Published: Springer 2018
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf
http://umpir.ump.edu.my/id/eprint/22276/
https://doi.org/10.1007/s12088-018-0755-8
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spelling my.ump.umpir.222762018-10-11T07:48:52Z http://umpir.ump.edu.my/id/eprint/22276/ Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro Lee, Kyung Dong Kuppusamy, Palaniselvam Kim, Da Hye Natanamurugaraj, Govindan Gaanty Pragas, Maniam Choi, Ki Choon Q Science (General) Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a green synthesis way. Potential antibacterial efficacy of these synthesized nanosized silver nanoparticles against poultry pathogenic bacteria was then studied. Aqueous extract of IRG was used as reducing agent for bio-reduction of silver salt to convert Ag+ to Ag0 metallic nano-silver. Size, shape, metallic composition, functional group, and crystalline nature of these synthesized silver nanoparticles were then characterized using UV–Vis spectrophotometer, FESEM, EDX, FT-IT, and XRD, respectively. In addition, antibacterial effects of these synthesized AgNPs against poultry pathogenic bacteria were evaluated by agar well diffusion method. UV–Vis spectra showed strong absorption peak of 440–450 nm with differ reaction time ranging from 30 min to 24 h. FESEM measurements revealed particles sizes of around 20–100 nm, majority of which were spherical in shape while a few were irregular. These biosynthesized silver nanoparticles using IRG extract exhibited strong antibacterial activities against poultry pathogenic microorganisms, including Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, and Bacillus subtilis. Overall results confirmed that IRG plant extract possessed potential bioactive compounds for converting silver ions into nanosized silver at room temperature without needing any external chemical for redox reaction. In addition, such synthesized AgNPs showed strong antibacterial activities against pathogenic bacteria responsible for infectious diseases in poultry. Springer 2018 Article PeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf Lee, Kyung Dong and Kuppusamy, Palaniselvam and Kim, Da Hye and Natanamurugaraj, Govindan and Gaanty Pragas, Maniam and Choi, Ki Choon (2018) Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro. Indian Journal of Microbiology, 58 (4). pp. 507-514. ISSN 0973-7715 https://doi.org/10.1007/s12088-018-0755-8 doi: 10.1007/s12088-018-0755-8
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Lee, Kyung Dong
Kuppusamy, Palaniselvam
Kim, Da Hye
Natanamurugaraj, Govindan
Gaanty Pragas, Maniam
Choi, Ki Choon
Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
description Italian ryegrass is one of main feed for livestock animals/birds. It has potential antioxidant metabolites that can improve their health and protect them against various infectious diseases. In this work, we studied synthesis of silver nanoparticles assisted by forage crop Lolium multiflorum as a green synthesis way. Potential antibacterial efficacy of these synthesized nanosized silver nanoparticles against poultry pathogenic bacteria was then studied. Aqueous extract of IRG was used as reducing agent for bio-reduction of silver salt to convert Ag+ to Ag0 metallic nano-silver. Size, shape, metallic composition, functional group, and crystalline nature of these synthesized silver nanoparticles were then characterized using UV–Vis spectrophotometer, FESEM, EDX, FT-IT, and XRD, respectively. In addition, antibacterial effects of these synthesized AgNPs against poultry pathogenic bacteria were evaluated by agar well diffusion method. UV–Vis spectra showed strong absorption peak of 440–450 nm with differ reaction time ranging from 30 min to 24 h. FESEM measurements revealed particles sizes of around 20–100 nm, majority of which were spherical in shape while a few were irregular. These biosynthesized silver nanoparticles using IRG extract exhibited strong antibacterial activities against poultry pathogenic microorganisms, including Pseudomonas aeruginosa, Salmonella typhi, Escherichia coli, and Bacillus subtilis. Overall results confirmed that IRG plant extract possessed potential bioactive compounds for converting silver ions into nanosized silver at room temperature without needing any external chemical for redox reaction. In addition, such synthesized AgNPs showed strong antibacterial activities against pathogenic bacteria responsible for infectious diseases in poultry.
format Article
author Lee, Kyung Dong
Kuppusamy, Palaniselvam
Kim, Da Hye
Natanamurugaraj, Govindan
Gaanty Pragas, Maniam
Choi, Ki Choon
author_facet Lee, Kyung Dong
Kuppusamy, Palaniselvam
Kim, Da Hye
Natanamurugaraj, Govindan
Gaanty Pragas, Maniam
Choi, Ki Choon
author_sort Lee, Kyung Dong
title Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_short Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_full Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_fullStr Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_full_unstemmed Forage Crop Lolium multiflorum Assisted Synthesis of AgNPs and Their Bioactivities Against Poultry Pathogenic Bacteria in In Vitro
title_sort forage crop lolium multiflorum assisted synthesis of agnps and their bioactivities against poultry pathogenic bacteria in in vitro
publisher Springer
publishDate 2018
url http://umpir.ump.edu.my/id/eprint/22276/1/Forage%20Crop%20Lolium%20multiflorum%20Assisted%20Synthesis%20of%20AgNPs%20and%20their%20bioactivities%20against%20poultry%20pathogenic%20bacteria%20in%20in%20vitro.pdf
http://umpir.ump.edu.my/id/eprint/22276/
https://doi.org/10.1007/s12088-018-0755-8
_version_ 1643669340006907904
score 13.211869

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