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Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant

Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and h...

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Main Authors: Abdul Aziz, Mohd Azoddein, Yana, Nuratri, Faten Ahada, Mohd Azli, Ahmad Bazli, Bustary
Format: Conference or Workshop Item
Language:English
Published: AIP Publishing 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/21436/1/1.5010534-1.pdf
http://umpir.ump.edu.my/id/eprint/21436/
https://doi.org/10.1063/1.5010534
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spelling my.ump.umpir.214362018-06-29T02:03:32Z http://umpir.ump.edu.my/id/eprint/21436/ Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant Abdul Aziz, Mohd Azoddein Yana, Nuratri Faten Ahada, Mohd Azli Ahmad Bazli, Bustary TP Chemical technology Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and high stability of the product. This study was aimed to freeze dry P. putida cells with addition of lyoprotectant. Lyoprotectant was added into the cells suspension prior to freezing. Dried P. putida obtained was then mixed with synthetic mercury. Viability of recovery P. putida after freeze dry was significantly influenced by the type of lyoprotectant. Among the lyoprotectants, tween 80/ sucrose was found to be the best lyoprotectant. Sucrose was able to recover more than 78% (6.2E+09 CFU/ml) of the original cells (7.90E+09CFU/ml) after freeze dry and able to retain 5.40E+05 viable cells after 4 weeks storage at 4 °C without vacuum. Polyethylene glycol (PEG) pre-treated freeze dried cells and broth pre-treated freeze dried cells after the freeze-dry process recovered more than 64% (5.0 E+09CFU/ml) and >0.1% (5.60E+07CFU/ml). Freeze-dried P. putida cells in PEG and broth cannot survive after 4 weeks storage. Freeze dry also does not really change the pattern of growth P. putida but extension of lag time was found 1 hour after 3 weeks of storage. Additional time was required for freeze-dried P. putida cells to recover before introducing freeze-dried cells to more complicated condition such as mercury solution. The maximum mercury reduction of PEG pre-treated freeze-dried cells after freeze dry and after storage of 3 weeks was 17.91 %. The maximum of mercury reduction of tween 80/sucrose pre-treated freeze-dried cells after freeze dry and after storage 3 weeks was 25.03%. Freeze dried P. putida was found to have lower mercury reduction compare to the fresh P. putida that has been grown in agar. Result from this study may be beneficial and useful as initial reference before commercialized freeze-dried P. putida. AIP Publishing 2017 Conference or Workshop Item PeerReviewed text en http://umpir.ump.edu.my/id/eprint/21436/1/1.5010534-1.pdf Abdul Aziz, Mohd Azoddein and Yana, Nuratri and Faten Ahada, Mohd Azli and Ahmad Bazli, Bustary (2017) Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant. In: AIP Conference Proceedings: Proceedings of the 3rd Advanced Materials Conference 2016 (3rd AMC 2016), 28-29 November 2016 , Langkawi, Malaysia. , 1901 (100012). ISBN 978-0-7354-1589-8 https://doi.org/10.1063/1.5010534
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abdul Aziz, Mohd Azoddein
Yana, Nuratri
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
description Pseudomonas putida is a potential strain in biological treatment to remove mercury contained in the effluent of petrochemical industry due to its mercury reductase enzyme that able to reduce ionic mercury to elementary mercury. Freeze-dried P. putida allows easy, inexpensive shipping, handling and high stability of the product. This study was aimed to freeze dry P. putida cells with addition of lyoprotectant. Lyoprotectant was added into the cells suspension prior to freezing. Dried P. putida obtained was then mixed with synthetic mercury. Viability of recovery P. putida after freeze dry was significantly influenced by the type of lyoprotectant. Among the lyoprotectants, tween 80/ sucrose was found to be the best lyoprotectant. Sucrose was able to recover more than 78% (6.2E+09 CFU/ml) of the original cells (7.90E+09CFU/ml) after freeze dry and able to retain 5.40E+05 viable cells after 4 weeks storage at 4 °C without vacuum. Polyethylene glycol (PEG) pre-treated freeze dried cells and broth pre-treated freeze dried cells after the freeze-dry process recovered more than 64% (5.0 E+09CFU/ml) and >0.1% (5.60E+07CFU/ml). Freeze-dried P. putida cells in PEG and broth cannot survive after 4 weeks storage. Freeze dry also does not really change the pattern of growth P. putida but extension of lag time was found 1 hour after 3 weeks of storage. Additional time was required for freeze-dried P. putida cells to recover before introducing freeze-dried cells to more complicated condition such as mercury solution. The maximum mercury reduction of PEG pre-treated freeze-dried cells after freeze dry and after storage of 3 weeks was 17.91 %. The maximum of mercury reduction of tween 80/sucrose pre-treated freeze-dried cells after freeze dry and after storage 3 weeks was 25.03%. Freeze dried P. putida was found to have lower mercury reduction compare to the fresh P. putida that has been grown in agar. Result from this study may be beneficial and useful as initial reference before commercialized freeze-dried P. putida.
format Conference or Workshop Item
author Abdul Aziz, Mohd Azoddein
Yana, Nuratri
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
author_facet Abdul Aziz, Mohd Azoddein
Yana, Nuratri
Faten Ahada, Mohd Azli
Ahmad Bazli, Bustary
author_sort Abdul Aziz, Mohd Azoddein
title Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
title_short Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
title_full Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
title_fullStr Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
title_full_unstemmed Assessing storage of stability and mercury reduction of freeze-dried Pseudomonas putida within different types of lyoprotectant
title_sort assessing storage of stability and mercury reduction of freeze-dried pseudomonas putida within different types of lyoprotectant
publisher AIP Publishing
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/21436/1/1.5010534-1.pdf
http://umpir.ump.edu.my/id/eprint/21436/
https://doi.org/10.1063/1.5010534
_version_ 1643669129587064832
score 13.211869

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