Staff View: Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)

Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)

The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in...

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Main Authors:	Rohaida, Che Man, R. M., Illias, Siti Zubaidah, Sulaiman, Siti Kholijah, Abdul Mudalip, Shalyda, Md Shaarani, Zatul Iffah, Mohd Arshad
Format:	Article
Language:	English
Published:	Bangladesh University of Engineering and Technology 2017
Subjects:	TP Chemical technology
Online Access:	http://umpir.ump.edu.my/id/eprint/20606/1/Effect%20of%20the%20cross%20linkers%E2%80%99%20concentration%20on%20the%20immobilization%20of%20recombinant%20Escherichia%20coli%20cells%20on%20hollow%20fiber%20membrane%20for%20excretion%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29.pdf http://umpir.ump.edu.my/id/eprint/20606/ https://www.banglajol.info/index.php/CERB/article/view/33810
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spelling	my.ump.umpir.206062018-02-23T02:48:23Z http://umpir.ump.edu.my/id/eprint/20606/ Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase) Rohaida, Che Man R. M., Illias Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad TP Chemical technology The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in the periplasmic space through overproduction of the expressed recombinant protein. Cell immobilization is a promising solution for the improvement of protein excretion with reduction in cell lysis. In this study, the effect of cross linkers concentrations on the process of cell immobilization for the cyclodextringlucanotransferase (CGTase) excretion and cell viability were investigated. The hollow fiber membrane was treated using different concentrations (0.3, 0.67 and 1%) of cross linkers namely glutaraldehyde (GA), poly-L-lysine (PLL) and polyethylenimine (PEI). The optimum concentration of cross linkers was found to be 0.3% with the high excretion of CGTase. Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% reduction of cell lysis, as compared to the immobilized cells on the treated hollow fiber membrane. It could be concluded that, the low concentration of cross linkers exhibited the highest CGTase excretion. Moreover, the non-treated hollow fiber membrane is a promising approach for attachment of cells to the membrane without requiring treatment with any chemicals for the production of recombinant enzyme. Bangladesh University of Engineering and Technology 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/20606/1/Effect%20of%20the%20cross%20linkers%E2%80%99%20concentration%20on%20the%20immobilization%20of%20recombinant%20Escherichia%20coli%20cells%20on%20hollow%20fiber%20membrane%20for%20excretion%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29.pdf Rohaida, Che Man and R. M., Illias and Siti Zubaidah, Sulaiman and Siti Kholijah, Abdul Mudalip and Shalyda, Md Shaarani and Zatul Iffah, Mohd Arshad (2017) Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase). Chemical Engineering Research Bulletin, 19 (Special). pp. 154-159. ISSN 0379-7678 (Print); 2072-9510 (Online) https://www.banglajol.info/index.php/CERB/article/view/33810 DOI: http://dx.doi.org/10.3329/cerb.v19i0.33810
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English
topic	TP Chemical technology
spellingShingle	TP Chemical technology Rohaida, Che Man R. M., Illias Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
description	The excretion of recombinant proteins into the culture medium is a preferred approach over cytoplasmic expression because of the high specific activity and ease of purification. However, cell lysis is one of a major problem during the excretion of recombinant protein due to the pressure build up in the periplasmic space through overproduction of the expressed recombinant protein. Cell immobilization is a promising solution for the improvement of protein excretion with reduction in cell lysis. In this study, the effect of cross linkers concentrations on the process of cell immobilization for the cyclodextringlucanotransferase (CGTase) excretion and cell viability were investigated. The hollow fiber membrane was treated using different concentrations (0.3, 0.67 and 1%) of cross linkers namely glutaraldehyde (GA), poly-L-lysine (PLL) and polyethylenimine (PEI). The optimum concentration of cross linkers was found to be 0.3% with the high excretion of CGTase. Interestingly, the immobilized cell on the non-treated hollow fiber membrane showed up to 15% increment of CGTase excretion with 55% reduction of cell lysis, as compared to the immobilized cells on the treated hollow fiber membrane. It could be concluded that, the low concentration of cross linkers exhibited the highest CGTase excretion. Moreover, the non-treated hollow fiber membrane is a promising approach for attachment of cells to the membrane without requiring treatment with any chemicals for the production of recombinant enzyme.
format	Article
author	Rohaida, Che Man R. M., Illias Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad
author_facet	Rohaida, Che Man R. M., Illias Siti Zubaidah, Sulaiman Siti Kholijah, Abdul Mudalip Shalyda, Md Shaarani Zatul Iffah, Mohd Arshad
author_sort	Rohaida, Che Man
title	Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
title_short	Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
title_full	Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
title_fullStr	Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
title_full_unstemmed	Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)
title_sort	effect of the cross linkers’ concentration on the immobilization of recombinant escherichia coli cells on hollow fiber membrane for excretion of cyclodextrin glucanotransferase (cgtase)
publisher	Bangladesh University of Engineering and Technology
publishDate	2017
url	http://umpir.ump.edu.my/id/eprint/20606/1/Effect%20of%20the%20cross%20linkers%E2%80%99%20concentration%20on%20the%20immobilization%20of%20recombinant%20Escherichia%20coli%20cells%20on%20hollow%20fiber%20membrane%20for%20excretion%20of%20cyclodextrin%20glucanotransferase%20%28CGTase%29.pdf http://umpir.ump.edu.my/id/eprint/20606/ https://www.banglajol.info/index.php/CERB/article/view/33810
_version_	1643668920748474368
score	13.211869

Effect of the Cross Linkers’ Concentration on the Immobilization of Recombinant Escherichia Coli Cells on Hollow Fiber Membrane for Excretion of Cyclodextrin Glucanotransferase (CGTase)

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