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Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma

SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a...

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Main Authors: Mahmood, Syed, Sengupta, Pinaki, Mandal, Uttam K., Chatterjee, Bappaditya, Taher, Muhammad
Format: Article
Language:English
Published: College of Pharmacists of Buenos Aires Province, Argentina 2017
Subjects:
RS Pharmacy and materia medica
Online Access:http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf
http://umpir.ump.edu.my/id/eprint/19791/
http://www.latamjpharm.org/resumenes/36/9/LAJOP_36_9_1_35.pdf
http://www.latamjpharm.org/previous_issue.php?vol=36&num=9
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spelling my.ump.umpir.197912018-02-05T02:31:17Z http://umpir.ump.edu.my/id/eprint/19791/ Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma Mahmood, Syed Sengupta, Pinaki Mandal, Uttam K. Chatterjee, Bappaditya Taher, Muhammad RS Pharmacy and materia medica SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific and stable. The applicability of the method was established through a single dose oral pharmacokinetic study of raloxifene in rat. College of Pharmacists of Buenos Aires Province, Argentina 2017-07 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf Mahmood, Syed and Sengupta, Pinaki and Mandal, Uttam K. and Chatterjee, Bappaditya and Taher, Muhammad (2017) Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma. Latin American Journal of Pharmacy, 36 (9). pp. 1901-1908. ISSN 2362-3853 http://www.latamjpharm.org/resumenes/36/9/LAJOP_36_9_1_35.pdf http://www.latamjpharm.org/previous_issue.php?vol=36&num=9
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic RS Pharmacy and materia medica
spellingShingle RS Pharmacy and materia medica
Mahmood, Syed
Sengupta, Pinaki
Mandal, Uttam K.
Chatterjee, Bappaditya
Taher, Muhammad
Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
description SUMMARY. A simple and sensitive HPLC method has been developed and validated for the quantification of raloxifene in rat plasma. Liquid- liquid extraction procedure was employed for extracting raloxifene from rat plasma sample. Separation of raloxifene was achieved through a RP- C18 column with a mobile phase consisted of phosphate buffer and acetonitrile (66:34 %, v/v). The method was validated for specificity, selectivity, sensitivity, linearity, accuracy, precision, recovery and stability parameters. A linear response (R2 value 0.9991) was found over the calibration range of 50 to 500 ng/mL. The accuracy for intra and inter day run varied between 86.73 to 102.30 % and 91.11 to 95.56%, respectively. The corresponding precision (% CV) were within 0.82 to 9.43% and 6.23 to 8.33%. The method was also found to be specific and stable. The applicability of the method was established through a single dose oral pharmacokinetic study of raloxifene in rat.
format Article
author Mahmood, Syed
Sengupta, Pinaki
Mandal, Uttam K.
Chatterjee, Bappaditya
Taher, Muhammad
author_facet Mahmood, Syed
Sengupta, Pinaki
Mandal, Uttam K.
Chatterjee, Bappaditya
Taher, Muhammad
author_sort Mahmood, Syed
title Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
title_short Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
title_full Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
title_fullStr Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
title_full_unstemmed Development, Validation and Pharmacokinetic Application of a Simple and Robust RP-HPLC Method for Quantitation of Raloxifene in Rat Plasma
title_sort development, validation and pharmacokinetic application of a simple and robust rp-hplc method for quantitation of raloxifene in rat plasma
publisher College of Pharmacists of Buenos Aires Province, Argentina
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/19791/1/ftech-2017-mahmood-Development%2C%20Validation%20and%20Pharmacokinetic%20Application1.pdf
http://umpir.ump.edu.my/id/eprint/19791/
http://www.latamjpharm.org/resumenes/36/9/LAJOP_36_9_1_35.pdf
http://www.latamjpharm.org/previous_issue.php?vol=36&num=9
_version_ 1643668735066636288
score 13.211869

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