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Synergistic ferulic acid production from banana stem waste by co-culture

Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacteria...

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Main Author: Kamaliah, Abdul Samad
Format: Thesis
Language:English
Published: 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf
http://umpir.ump.edu.my/id/eprint/19559/
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spelling my.ump.umpir.195592022-01-04T23:38:34Z http://umpir.ump.edu.my/id/eprint/19559/ Synergistic ferulic acid production from banana stem waste by co-culture Kamaliah, Abdul Samad TP Chemical technology Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacterial co-culture fermentation technique using banana stem waste (BSW) as substrate was implemented to improve FA production. Soil microbes were isolated and preliminary study was performed to select the best bacterial strain. Subsequently, 26 type of bacterial co-cultures were formed and evaluated their ability dependent on FA production. Optimization of the FA production was conducted by employing response surface methodology using the selected co-culture as inoculum. A kinetic study was carried out to determine kinetic constants using Michaelis-Menten equation. Meanwhile, the enzyme assay was performed to investigate the mechanism release of FA by enzymatic hydrolysis. Ferulic acid production was accomplished through submerged fermentation and the sample was analyzed by HPLC to quantify the FA content. The result found that 5 out of 46 isolated bacterial strains were efficient in releasing FA from BSW. However, the highest production of FA was observed in co-culture A (Bacillus cereus CCM 2010, Bacillus pumilus SAFR-032 and Bacillus thuringiensis Bt407), followed by co-culture B (Bacillus cereus CCM 2010, and Bacillus thuringiensis Bt407). In improving the fermentation process, screening by fractional factorial design was performed and the result obtained presented four factors including pH, rotation rate, type of co-culture, and volume of inoculum have significant effects on FA production. Furthermore, rotation rate at 150 rpm and volume of inoculum of 5 % were found to be optimum in increasing FA yield up to 510.24 mg/kg within 24 h using bacterial co-culture B as an inoculum. Meanwhile, the kinetic constant of 2017-05 Thesis NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf Kamaliah, Abdul Samad (2017) Synergistic ferulic acid production from banana stem waste by co-culture. PhD thesis, Universiti Malaysia Pahang.
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Kamaliah, Abdul Samad
Synergistic ferulic acid production from banana stem waste by co-culture
description Ferulic acid (FA) production using microbes has currently become extensive owing to its antioxidant properties. Both fungi and bacteria have been used in producing ferulic acid from agricultural waste. However, less work has been done on bacteria compared to fungi. Therefore, an appropriate bacterial co-culture fermentation technique using banana stem waste (BSW) as substrate was implemented to improve FA production. Soil microbes were isolated and preliminary study was performed to select the best bacterial strain. Subsequently, 26 type of bacterial co-cultures were formed and evaluated their ability dependent on FA production. Optimization of the FA production was conducted by employing response surface methodology using the selected co-culture as inoculum. A kinetic study was carried out to determine kinetic constants using Michaelis-Menten equation. Meanwhile, the enzyme assay was performed to investigate the mechanism release of FA by enzymatic hydrolysis. Ferulic acid production was accomplished through submerged fermentation and the sample was analyzed by HPLC to quantify the FA content. The result found that 5 out of 46 isolated bacterial strains were efficient in releasing FA from BSW. However, the highest production of FA was observed in co-culture A (Bacillus cereus CCM 2010, Bacillus pumilus SAFR-032 and Bacillus thuringiensis Bt407), followed by co-culture B (Bacillus cereus CCM 2010, and Bacillus thuringiensis Bt407). In improving the fermentation process, screening by fractional factorial design was performed and the result obtained presented four factors including pH, rotation rate, type of co-culture, and volume of inoculum have significant effects on FA production. Furthermore, rotation rate at 150 rpm and volume of inoculum of 5 % were found to be optimum in increasing FA yield up to 510.24 mg/kg within 24 h using bacterial co-culture B as an inoculum. Meanwhile, the kinetic constant of
format Thesis
author Kamaliah, Abdul Samad
author_facet Kamaliah, Abdul Samad
author_sort Kamaliah, Abdul Samad
title Synergistic ferulic acid production from banana stem waste by co-culture
title_short Synergistic ferulic acid production from banana stem waste by co-culture
title_full Synergistic ferulic acid production from banana stem waste by co-culture
title_fullStr Synergistic ferulic acid production from banana stem waste by co-culture
title_full_unstemmed Synergistic ferulic acid production from banana stem waste by co-culture
title_sort synergistic ferulic acid production from banana stem waste by co-culture
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/19559/19/Synergistic%20ferulic%20acid%20production%20from%20banana%20stem%20waste%20by%20co-culture.pdf
http://umpir.ump.edu.my/id/eprint/19559/
_version_ 1724073467515502592
score 13.211869

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