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Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology

The interaction between incubation temperature, yeast extract concentration and inoculum size was investigated to optimize critical environmental parameters for production of biobutanol from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824 using response surface methodology (RSM). A...

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Main Authors: Nur Syazana, Muhamad Nasrah, Mior Ahmad Khushairi, Mohd Zahari, N., Masngut, Hidayah, Ariffin
Format: Conference or Workshop Item
Language:English
Published: EDP Sciences 2017
Subjects:
TP Chemical technology
Online Access:http://umpir.ump.edu.my/id/eprint/18685/1/Statistical%20Optimization%20for%20Biobutanol%20Production%20by%20Clostridium%20acetobutylicum%20ATCC%20824%20from%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20Using%20Response%20Surface%20Methodology.pdf
http://umpir.ump.edu.my/id/eprint/18685/
https://doi.org/10.1051/matecconf/201711103001
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spelling my.ump.umpir.186852018-03-07T01:52:35Z http://umpir.ump.edu.my/id/eprint/18685/ Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology Nur Syazana, Muhamad Nasrah Mior Ahmad Khushairi, Mohd Zahari N., Masngut Hidayah, Ariffin TP Chemical technology The interaction between incubation temperature, yeast extract concentration and inoculum size was investigated to optimize critical environmental parameters for production of biobutanol from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824 using response surface methodology (RSM). A central composite design (CCD) was applied as the experimental design and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for the biobutanol production. The incubation temperature, yeast extract concentration and inoculum size showed significant value at p < 0.005. The results of optimization process showed that a maximum biobutanol production was obtained under the condition of temperature 37 °C, yeast extract concentration 5.5 g/L and inoculum size 10%. Under these optimized conditions, the highest biobutanol yield was 0.3054 g/g after 144 hours of incubation period. The model was validated by applying the optimized conditions and 0.2992 g/g biobutanol yield was obtained. These experimental findings were in close agreement with the model prediction, with a difference of only 9.76%. EDP Sciences 2017 Conference or Workshop Item PeerReviewed application/pdf en cc_by http://umpir.ump.edu.my/id/eprint/18685/1/Statistical%20Optimization%20for%20Biobutanol%20Production%20by%20Clostridium%20acetobutylicum%20ATCC%20824%20from%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20Using%20Response%20Surface%20Methodology.pdf Nur Syazana, Muhamad Nasrah and Mior Ahmad Khushairi, Mohd Zahari and N., Masngut and Hidayah, Ariffin (2017) Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology. In: MATEC Web of Conferences: Fluids and Chemical Engineering Conference (FluidsChE 2017) , 4-6 April 2017 , Kota Kinabalu, Sabah, Malaysia. pp. 1-8., 111 (03001). ISSN 2261-236X https://doi.org/10.1051/matecconf/201711103001 DOI: 10.1051/matecconf/20171110
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Nur Syazana, Muhamad Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
description The interaction between incubation temperature, yeast extract concentration and inoculum size was investigated to optimize critical environmental parameters for production of biobutanol from oil palm frond (OPF) juice by Clostridium acetobutylicum ATCC 824 using response surface methodology (RSM). A central composite design (CCD) was applied as the experimental design and a polynomial regression model with quadratic term was used to analyse the experimental data using analysis of variance (ANOVA). ANOVA analysis showed that the model was very significant (p < 0.0001) for the biobutanol production. The incubation temperature, yeast extract concentration and inoculum size showed significant value at p < 0.005. The results of optimization process showed that a maximum biobutanol production was obtained under the condition of temperature 37 °C, yeast extract concentration 5.5 g/L and inoculum size 10%. Under these optimized conditions, the highest biobutanol yield was 0.3054 g/g after 144 hours of incubation period. The model was validated by applying the optimized conditions and 0.2992 g/g biobutanol yield was obtained. These experimental findings were in close agreement with the model prediction, with a difference of only 9.76%.
format Conference or Workshop Item
author Nur Syazana, Muhamad Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
author_facet Nur Syazana, Muhamad Nasrah
Mior Ahmad Khushairi, Mohd Zahari
N., Masngut
Hidayah, Ariffin
author_sort Nur Syazana, Muhamad Nasrah
title Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
title_short Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
title_full Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
title_fullStr Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
title_full_unstemmed Statistical Optimization for Biobutanol Production by Clostridium acetobutylicum ATCC 824 from Oil Palm Frond (OPF) Juice Using Response Surface Methodology
title_sort statistical optimization for biobutanol production by clostridium acetobutylicum atcc 824 from oil palm frond (opf) juice using response surface methodology
publisher EDP Sciences
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/18685/1/Statistical%20Optimization%20for%20Biobutanol%20Production%20by%20Clostridium%20acetobutylicum%20ATCC%20824%20from%20Oil%20Palm%20Frond%20%28OPF%29%20Juice%20Using%20Response%20Surface%20Methodology.pdf
http://umpir.ump.edu.my/id/eprint/18685/
https://doi.org/10.1051/matecconf/201711103001
_version_ 1643668509475995648
score 13.211869

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