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Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance

Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received consid...

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Main Authors: Nurhusna, Samsudin, Yumi Zuhanis Has-Yun, Hashim, Mohd Azmir, Arifin, Maizirwan, Mel, Hamzah, Mohd Salleh, Iis, Sopyan, Dzun Noraini, Jimat
Format: Article
Language:English
Published: Springer International Publishing 2017
Subjects:
T Technology (General)
Online Access:http://umpir.ump.edu.my/id/eprint/17479/2/Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone%20%28PCL%29%20microcarrier%20performance%201.pdf
http://umpir.ump.edu.my/id/eprint/17479/
https://doi.org/10.1007/s10616-017-0071-x
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spelling my.ump.umpir.174792018-06-28T01:37:43Z http://umpir.ump.edu.my/id/eprint/17479/ Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance Nurhusna, Samsudin Yumi Zuhanis Has-Yun, Hashim Mohd Azmir, Arifin Maizirwan, Mel Hamzah, Mohd Salleh Iis, Sopyan Dzun Noraini, Jimat T Technology (General) Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 µg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93°–49.34°) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL. Springer International Publishing 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/17479/2/Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone%20%28PCL%29%20microcarrier%20performance%201.pdf Nurhusna, Samsudin and Yumi Zuhanis Has-Yun, Hashim and Mohd Azmir, Arifin and Maizirwan, Mel and Hamzah, Mohd Salleh and Iis, Sopyan and Dzun Noraini, Jimat (2017) Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance. Cytotechnology, 69 (4). pp. 601-616. ISSN 0920-9069 (Print); 1573-0778 (Online) https://doi.org/10.1007/s10616-017-0071-x DOI: 10.1007/s10616-017-0071-x
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic T Technology (General)
spellingShingle T Technology (General)
Nurhusna, Samsudin
Yumi Zuhanis Has-Yun, Hashim
Mohd Azmir, Arifin
Maizirwan, Mel
Hamzah, Mohd Salleh
Iis, Sopyan
Dzun Noraini, Jimat
Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
description Growing cells on microcarriers may have overcome the limitation of conventional cell culture system. However, the surface functionality of certain polymeric microcarriers for effective cell attachment and growth remains a challenge. Polycaprolactone (PCL), a biodegradable polymer has received considerable attention due to its good mechanical properties and degradation rate. The drawback is the non-polar hydrocarbon moiety which makes it not readily suitable for cell attachment. This report concerns the modification of PCL microcarrier surface (introduction of functional oxygen groups) using ultraviolet irradiation and ozone (UV/O3) system and investigation of the effects of ozone concentration, the amount of PCL and exposure time; where the optimum conditions were found to be at 60,110.52 ppm, 5.5 g PCL and 60 min, respectively. The optimum concentration of carboxyl group (COOH) absorbed on the surface was 1495.92 nmol/g and the amount of gelatin immobilized was 320 ± 0.9 µg/g on UV/O3 treated microcarriers as compared to the untreated (26.83 ± 3 µg/g) microcarriers. The absorption of functional oxygen groups on the surface and the immobilized gelatin was confirmed with the attenuated total reflectance Fourier transformed infrared spectroscopy (ATR-FTIR) and the enhancement of hydrophilicity of the surface was confirmed using water contact angle measurement which decreased (86.93°–49.34°) after UV/O3 treatment and subsequently after immobilization of gelatin. The attachment and growth kinetics for HaCaT skin keratinocyte cells showed that adhesion occurred much more rapidly for oxidized surfaces and gelatin immobilized surface as compared to untreated PCL.
format Article
author Nurhusna, Samsudin
Yumi Zuhanis Has-Yun, Hashim
Mohd Azmir, Arifin
Maizirwan, Mel
Hamzah, Mohd Salleh
Iis, Sopyan
Dzun Noraini, Jimat
author_facet Nurhusna, Samsudin
Yumi Zuhanis Has-Yun, Hashim
Mohd Azmir, Arifin
Maizirwan, Mel
Hamzah, Mohd Salleh
Iis, Sopyan
Dzun Noraini, Jimat
author_sort Nurhusna, Samsudin
title Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
title_short Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
title_full Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
title_fullStr Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
title_full_unstemmed Optimization of Ultraviolet Ozone Treatment Process for Improvement of Polycaprolactone (PCL) Microcarrier Performance
title_sort optimization of ultraviolet ozone treatment process for improvement of polycaprolactone (pcl) microcarrier performance
publisher Springer International Publishing
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/17479/2/Optimization%20of%20ultraviolet%20ozone%20treatment%20process%20for%20improvement%20of%20polycaprolactone%20%28PCL%29%20microcarrier%20performance%201.pdf
http://umpir.ump.edu.my/id/eprint/17479/
https://doi.org/10.1007/s10616-017-0071-x
_version_ 1643668193308311552
score 13.211869

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