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Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks

We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of...

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Main Authors: A. K. M., Kafi, N. S., Azmi, M. M., Yusoff, Crossley, Maxwell J.
Format: Article
Language:English
Published: American Scientific Publishers 2017
Subjects:
QD Chemistry
Online Access:http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf
http://umpir.ump.edu.my/id/eprint/15616/
http://www.ingentaconnect.com/content/asp/jnn/2017/00000017/00000008/art00128
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spelling my.ump.umpir.156162018-07-26T03:23:49Z http://umpir.ump.edu.my/id/eprint/15616/ Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks A. K. M., Kafi N. S., Azmi M. M., Yusoff Crossley, Maxwell J. QD Chemistry We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V, cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide. American Scientific Publishers 2017 Article PeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf A. K. M., Kafi and N. S., Azmi and M. M., Yusoff and Crossley, Maxwell J. (2017) Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks. Journal of Nanoscience and Nanotechnology, 17 (8). pp. 5896-5899. ISSN 1533-4880 (Print); 1533-4899 (Online) http://www.ingentaconnect.com/content/asp/jnn/2017/00000017/00000008/art00128 doi: 10.1166/jnn.2017.13860
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic QD Chemistry
spellingShingle QD Chemistry
A. K. M., Kafi
N. S., Azmi
M. M., Yusoff
Crossley, Maxwell J.
Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
description We report on a novel 3-dimensional (3D) network of crosslinked Cytochrome C/Carbon Nanotube (CytC/CNT) on a thiol-modified gold surface which can establish direct electrical communication between the redox center of Cytochrome C and the electrode. Cyclic voltammograms (CVs) results showed a pair of well defined redox peaks for Cytochrome C, located at about −0.03 and +0.06 V, cathodic and anodic respectively. Additionally, the formal potential E0 of adsorbed Cyt c was found to be 15 mV, a value close to that of native Cyt c. Based on 3D Cytochrome c and carbon nanotube network, a sensitive superoxide radical biosensor has been proposed. The biosensor showed high sensitivity and lower detection limit of 0.3 �M of superoxide.
format Article
author A. K. M., Kafi
N. S., Azmi
M. M., Yusoff
Crossley, Maxwell J.
author_facet A. K. M., Kafi
N. S., Azmi
M. M., Yusoff
Crossley, Maxwell J.
author_sort A. K. M., Kafi
title Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
title_short Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
title_full Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
title_fullStr Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
title_full_unstemmed Superoxide Radical Biosensor Based on a 3D Enzyme/Carbon Nanotube Conductive Networks
title_sort superoxide radical biosensor based on a 3d enzyme/carbon nanotube conductive networks
publisher American Scientific Publishers
publishDate 2017
url http://umpir.ump.edu.my/id/eprint/15616/1/fist-2017-kafi-Superoxide%20Radical%20Biosensor%20Based1.pdf
http://umpir.ump.edu.my/id/eprint/15616/
http://www.ingentaconnect.com/content/asp/jnn/2017/00000017/00000008/art00128
_version_ 1643667719874150400
score 13.211869

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