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Application of enzyme-producing bacteria for municipal solid waste biodegradation

Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were or...

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Main Author: Nailah, Sa’adah
Format: Thesis
Language:English
Published: 2015
Subjects:
Q Science (General)
Online Access:http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf
http://umpir.ump.edu.my/id/eprint/12615/
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spelling my.ump.umpir.126152023-04-10T07:25:46Z http://umpir.ump.edu.my/id/eprint/12615/ Application of enzyme-producing bacteria for municipal solid waste biodegradation Nailah, Sa’adah Q Science (General) Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. Meanwhile, the compositions of commercial waste are food, plastic, yard waste, paper, cardboard, textile, glass, non-ferrous, rubber, wood, ferrous metals. Landfill system always produces leachate. This waste contains many types of bacteria with the potential to degrade the waste compound. Screening tests with selective media demonstrated the ability of bacteria to produce amylase, lipase, protease and cellulase enzymes. These enzymes are needed to accelerate the molecules breakdown of municipal solid waste in the biodegradation process. Samples for isolation of bacteria were taken from different places, namely landfill soil and leachate. Identification of bacteria was conducted using Gen III microplate BIOLOG microbial identification system. They were Bacillus amyloliquefaciens, Bacillus ruris, Bacillus licheniformis, Bacillus subtilis and Kocuria varians. There were 4 different treatments: composting pile without bacteria (T0), composting pile with inoculation of amylase and protease producing bacteria (T1), composting with inoculation of lipase and cellulase producing bacteria (T2), and composting with inoculation all enzyme-producing bacteria (T3). The result of biodegradation process of T3 treatment reached the highest temperature (53° C) with the longest thermophilic phase compared to other treatments. The lower value of C/N ratio, the more stable the level of maturity of compost. The lowest of C/N ratio value was T3 (10%). T3 treatment compared with other treatments can increase as much as 27% content of nitrogen, 67% of phosphorus and 33% of potassium. All the treatments with bacterial inoculation (T1, T2, and T3) are able to reduce the content of heavy metals (Fe, Zn, Cu) on municipal solid waste biodegradation. It can be concluded that the inoculation of potential enzyme-producing of bacteria on municipal solid waste biodegradation is effective to increase the nutrient content and decrease the heavy metals. 2015-08 Thesis NonPeerReviewed pdf en http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf Nailah, Sa’adah (2015) Application of enzyme-producing bacteria for municipal solid waste biodegradation. Masters thesis, Universiti Malaysia Pahang (Contributors, Thesis advisor: Wan Ishak, Wan Mohd Faizal).
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic Q Science (General)
spellingShingle Q Science (General)
Nailah, Sa’adah
Application of enzyme-producing bacteria for municipal solid waste biodegradation
description Population growth with increasing consumption levels leads to abundant waste in Kuantan. Jabor landfill, commonly known as Kuantan landfill, receives more than 500 tons of waste per day with a composition of 60% of domestic waste and 40% of commercial waste. The composition of domestic waste were organic waste, green waste, mixed paper, plastic, textile, ferrous, glass, rubber and leather, and others. Meanwhile, the compositions of commercial waste are food, plastic, yard waste, paper, cardboard, textile, glass, non-ferrous, rubber, wood, ferrous metals. Landfill system always produces leachate. This waste contains many types of bacteria with the potential to degrade the waste compound. Screening tests with selective media demonstrated the ability of bacteria to produce amylase, lipase, protease and cellulase enzymes. These enzymes are needed to accelerate the molecules breakdown of municipal solid waste in the biodegradation process. Samples for isolation of bacteria were taken from different places, namely landfill soil and leachate. Identification of bacteria was conducted using Gen III microplate BIOLOG microbial identification system. They were Bacillus amyloliquefaciens, Bacillus ruris, Bacillus licheniformis, Bacillus subtilis and Kocuria varians. There were 4 different treatments: composting pile without bacteria (T0), composting pile with inoculation of amylase and protease producing bacteria (T1), composting with inoculation of lipase and cellulase producing bacteria (T2), and composting with inoculation all enzyme-producing bacteria (T3). The result of biodegradation process of T3 treatment reached the highest temperature (53° C) with the longest thermophilic phase compared to other treatments. The lower value of C/N ratio, the more stable the level of maturity of compost. The lowest of C/N ratio value was T3 (10%). T3 treatment compared with other treatments can increase as much as 27% content of nitrogen, 67% of phosphorus and 33% of potassium. All the treatments with bacterial inoculation (T1, T2, and T3) are able to reduce the content of heavy metals (Fe, Zn, Cu) on municipal solid waste biodegradation. It can be concluded that the inoculation of potential enzyme-producing of bacteria on municipal solid waste biodegradation is effective to increase the nutrient content and decrease the heavy metals.
format Thesis
author Nailah, Sa’adah
author_facet Nailah, Sa’adah
author_sort Nailah, Sa’adah
title Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_short Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_full Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_fullStr Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_full_unstemmed Application of enzyme-producing bacteria for municipal solid waste biodegradation
title_sort application of enzyme-producing bacteria for municipal solid waste biodegradation
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/12615/1/Application%20of%20enzyme-producing%20bacteria%20for%20municipal%20solid%20waste%20biodegradation.pdf
http://umpir.ump.edu.my/id/eprint/12615/
_version_ 1762837608373682176
score 13.211869

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