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Extraction of Keratin Protein from Chicken Feather

The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products. Chicken feathers have high level of keratin protein content and can become a suit...

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Main Authors:	Gupta, Arun, Nuruldiyanah, Kamarudin, Chua, Gek Kee, R. M., Yunus
Format:	Article
Language:	English
Published:	David Publishing 2012
Subjects:	Q Science (General) T Technology (General)
Online Access:	http://umpir.ump.edu.my/id/eprint/11965/1/Keratin%20protein%20from%20chicken%20feather.pdf http://umpir.ump.edu.my/id/eprint/11965/ http://www.davidpublishing.com/davidpublishing/upfile/10/15/2012/2012101571362425.pdf
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spelling	my.ump.umpir.119652018-01-15T04:04:47Z http://umpir.ump.edu.my/id/eprint/11965/ Extraction of Keratin Protein from Chicken Feather Gupta, Arun Nuruldiyanah, Kamarudin Chua, Gek Kee R. M., Yunus Q Science (General) T Technology (General) The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products. Chicken feathers have high level of keratin protein content and can become a suitable protein source. The main processes involved are first dissolving chicken feat hers using different reducing agents and later on separating the protein from chemicals. Reducing agents used are potassium cyanide, thioglycolic acid and sodium sulphide. Once the feathers are dissolved using reducing agents, ammonium sulfate solution is added to the solution for the precipitation of protein. The precipitated protein is washed with water several times and sodium hydroxide solution is used to obtain protein back in the solution form. Out of three different reducing agents used, sodium sulfide gives the highest efficiency in dissolving chicken feathers since the feathers are dissolved in a very short period of time. The percentage of keratin protein is evaluated by means of biuret test and FTIR analysis. The analysis by FTIR confirmed the presence of carboxyl acid and amino groups in the protein solution. The biuret test he lps in determining the concentration of protein obtained from different methods. Thus these two tests confirm the presence of protein in the solution. From this research, it can be concluded that protein can be extracted from chicken feathers. The keratin protein solution can be used for several purposes such as anti-aging cream, shampoo, and conditioner and for medical purposes such as bone replacement and bone graft. David Publishing 2012 Article NonPeerReviewed application/pdf en http://umpir.ump.edu.my/id/eprint/11965/1/Keratin%20protein%20from%20chicken%20feather.pdf Gupta, Arun and Nuruldiyanah, Kamarudin and Chua, Gek Kee and R. M., Yunus (2012) Extraction of Keratin Protein from Chicken Feather. Journal of Chemistry and Chemical Engineering, 6 (8). pp. 732-737. ISSN 1934-7375 http://www.davidpublishing.com/davidpublishing/upfile/10/15/2012/2012101571362425.pdf
institution	Universiti Malaysia Pahang
building	UMP Library
collection	Institutional Repository
continent	Asia
country	Malaysia
content_provider	Universiti Malaysia Pahang
content_source	UMP Institutional Repository
url_provider	http://umpir.ump.edu.my/
language	English
topic	Q Science (General) T Technology (General)
spellingShingle	Q Science (General) T Technology (General) Gupta, Arun Nuruldiyanah, Kamarudin Chua, Gek Kee R. M., Yunus Extraction of Keratin Protein from Chicken Feather
description	The present research was conducted to extract keratin protein from chicken feathers. Protein is an important nutrient needed by our body to maintain body structures and is an important ingredient for cosmetic products. Chicken feathers have high level of keratin protein content and can become a suitable protein source. The main processes involved are first dissolving chicken feat hers using different reducing agents and later on separating the protein from chemicals. Reducing agents used are potassium cyanide, thioglycolic acid and sodium sulphide. Once the feathers are dissolved using reducing agents, ammonium sulfate solution is added to the solution for the precipitation of protein. The precipitated protein is washed with water several times and sodium hydroxide solution is used to obtain protein back in the solution form. Out of three different reducing agents used, sodium sulfide gives the highest efficiency in dissolving chicken feathers since the feathers are dissolved in a very short period of time. The percentage of keratin protein is evaluated by means of biuret test and FTIR analysis. The analysis by FTIR confirmed the presence of carboxyl acid and amino groups in the protein solution. The biuret test he lps in determining the concentration of protein obtained from different methods. Thus these two tests confirm the presence of protein in the solution. From this research, it can be concluded that protein can be extracted from chicken feathers. The keratin protein solution can be used for several purposes such as anti-aging cream, shampoo, and conditioner and for medical purposes such as bone replacement and bone graft.
format	Article
author	Gupta, Arun Nuruldiyanah, Kamarudin Chua, Gek Kee R. M., Yunus
author_facet	Gupta, Arun Nuruldiyanah, Kamarudin Chua, Gek Kee R. M., Yunus
author_sort	Gupta, Arun
title	Extraction of Keratin Protein from Chicken Feather
title_short	Extraction of Keratin Protein from Chicken Feather
title_full	Extraction of Keratin Protein from Chicken Feather
title_fullStr	Extraction of Keratin Protein from Chicken Feather
title_full_unstemmed	Extraction of Keratin Protein from Chicken Feather
title_sort	extraction of keratin protein from chicken feather
publisher	David Publishing
publishDate	2012
url	http://umpir.ump.edu.my/id/eprint/11965/1/Keratin%20protein%20from%20chicken%20feather.pdf http://umpir.ump.edu.my/id/eprint/11965/ http://www.davidpublishing.com/davidpublishing/upfile/10/15/2012/2012101571362425.pdf
_version_	1643666782517460992
score	13.211869

Extraction of Keratin Protein from Chicken Feather

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