Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor

Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum based industrial plants had showed high concentration of mercury compare to the Department of Environmental (DOE), Malaysia. Microbes have been used to solve environmental wastewater pr...

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Main Authors: Abdul Aziz, Mohd Azoddein, R. A., Bakar, Nik Meriam, Nik Sulaiman, Ahmad Bazli, Bustary, Nur Athirah, Mohamad Basir
Format: Conference or Workshop Item
Language:English
Published: 2015
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Online Access:http://umpir.ump.edu.my/id/eprint/10704/1/Mercury%20Removal%20from%20Actual%20Petroleum%20Based%20Industries%20Wastewater%20by%20P.%20putida%20in%20Membrane%20Bioreactor.pdf
http://umpir.ump.edu.my/id/eprint/10704/
http://www.globalilluminators.org/ndmrp-2015-turkey/full-paper-proceding-ndmrp-2015-turkey/
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spelling my.ump.umpir.107042020-02-28T02:22:09Z http://umpir.ump.edu.my/id/eprint/10704/ Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor Abdul Aziz, Mohd Azoddein R. A., Bakar Nik Meriam, Nik Sulaiman Ahmad Bazli, Bustary Nur Athirah, Mohamad Basir TP Chemical technology Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum based industrial plants had showed high concentration of mercury compare to the Department of Environmental (DOE), Malaysia. Microbes have been used to solve environmental wastewater problems for many years. Objective of this study is to remove mercury from actual petrochemical wastewater using Pseudomonas putida (P. putida), in membrane bioreactor. To achieve the maximum of mercury removal, the optimum growth parameters of P. putida were obtained. Based on the optimum parameters of P. putida for specific growth rate, µ the removal of 4 mg/L was studied. Results showed mercury removal for sample with 4 mg/L mercury in bioreactor is 99.60% for the first 6 hours, 99.80% removal for 120 hours and 99.90 % after the microfiltration membrane system. The specific growth rate (μ) describes how fast the cells are reproducing. The higher the value of specific growth rate, then the faster the cells are growing.In this case, 6 hours was the optimum time for the mercury removal with the ratio of mercury mass over cell mass is 20.78 µgHg/g cells for P. putida. Microfiltration membrane enhanced further the treatment of the wastewater by retaining the P. putida from escaping during the release of treated wastewater, reducing the turbidity by 94.2% (5.32 NTU) and concentration of suspended solids up to 60.4% (0.09 mg/L). The mechanism of mercury detoxification in the membrane bioreactor was based on reduction of Hg²⁺ to non-toxic Hg0 by mercury reductase enzyme produced by P. putida. The findings from this study can be used as references for future application of petroleum based industries wastewater treatment as well as other industries related to mercury contamination in their wastewater treatment plant such as gold mining, chemical industries, agriculture etc. 2015 Conference or Workshop Item PeerReviewed application/pdf en cc_by_nc_nd http://umpir.ump.edu.my/id/eprint/10704/1/Mercury%20Removal%20from%20Actual%20Petroleum%20Based%20Industries%20Wastewater%20by%20P.%20putida%20in%20Membrane%20Bioreactor.pdf Abdul Aziz, Mohd Azoddein and R. A., Bakar and Nik Meriam, Nik Sulaiman and Ahmad Bazli, Bustary and Nur Athirah, Mohamad Basir (2015) Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor. In: New Directions in Multidisciplinary Research & Practice (NDMRP 2015), 12-13 May 2015 , Istanbul, Turkey. pp. 313-330.. http://www.globalilluminators.org/ndmrp-2015-turkey/full-paper-proceding-ndmrp-2015-turkey/
institution Universiti Malaysia Pahang
building UMP Library
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Pahang
content_source UMP Institutional Repository
url_provider http://umpir.ump.edu.my/
language English
topic TP Chemical technology
spellingShingle TP Chemical technology
Abdul Aziz, Mohd Azoddein
R. A., Bakar
Nik Meriam, Nik Sulaiman
Ahmad Bazli, Bustary
Nur Athirah, Mohamad Basir
Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
description Mercury is an extremely toxic pollutant that currently being emitted and distributed globally. Several petroleum based industrial plants had showed high concentration of mercury compare to the Department of Environmental (DOE), Malaysia. Microbes have been used to solve environmental wastewater problems for many years. Objective of this study is to remove mercury from actual petrochemical wastewater using Pseudomonas putida (P. putida), in membrane bioreactor. To achieve the maximum of mercury removal, the optimum growth parameters of P. putida were obtained. Based on the optimum parameters of P. putida for specific growth rate, µ the removal of 4 mg/L was studied. Results showed mercury removal for sample with 4 mg/L mercury in bioreactor is 99.60% for the first 6 hours, 99.80% removal for 120 hours and 99.90 % after the microfiltration membrane system. The specific growth rate (μ) describes how fast the cells are reproducing. The higher the value of specific growth rate, then the faster the cells are growing.In this case, 6 hours was the optimum time for the mercury removal with the ratio of mercury mass over cell mass is 20.78 µgHg/g cells for P. putida. Microfiltration membrane enhanced further the treatment of the wastewater by retaining the P. putida from escaping during the release of treated wastewater, reducing the turbidity by 94.2% (5.32 NTU) and concentration of suspended solids up to 60.4% (0.09 mg/L). The mechanism of mercury detoxification in the membrane bioreactor was based on reduction of Hg²⁺ to non-toxic Hg0 by mercury reductase enzyme produced by P. putida. The findings from this study can be used as references for future application of petroleum based industries wastewater treatment as well as other industries related to mercury contamination in their wastewater treatment plant such as gold mining, chemical industries, agriculture etc.
format Conference or Workshop Item
author Abdul Aziz, Mohd Azoddein
R. A., Bakar
Nik Meriam, Nik Sulaiman
Ahmad Bazli, Bustary
Nur Athirah, Mohamad Basir
author_facet Abdul Aziz, Mohd Azoddein
R. A., Bakar
Nik Meriam, Nik Sulaiman
Ahmad Bazli, Bustary
Nur Athirah, Mohamad Basir
author_sort Abdul Aziz, Mohd Azoddein
title Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
title_short Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
title_full Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
title_fullStr Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
title_full_unstemmed Mercury Removal from Actual Petroleum Based Industries Wastewater by P. putida in Membrane Bioreactor
title_sort mercury removal from actual petroleum based industries wastewater by p. putida in membrane bioreactor
publishDate 2015
url http://umpir.ump.edu.my/id/eprint/10704/1/Mercury%20Removal%20from%20Actual%20Petroleum%20Based%20Industries%20Wastewater%20by%20P.%20putida%20in%20Membrane%20Bioreactor.pdf
http://umpir.ump.edu.my/id/eprint/10704/
http://www.globalilluminators.org/ndmrp-2015-turkey/full-paper-proceding-ndmrp-2015-turkey/
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score 13.211869