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Preliminary study on leech crude extract as an anti-coagulant agent

Hirudin is the generic name for a family of closely related homologous peptides that are all found in the cranial salivary glands of medicinal leech (Wallis, 1996). In 1984, the blood-thinning agent (anticoagulant) hirudin was isolated for the first time from leech salivary glands (Weinnberg, 1994)....

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Main Authors: Zulhisyam Abdul Kari @ Abdullah, Ahmad Anwar Mohamed Ismail, Ibrahim Che Omar
Format: Conference or Workshop Item
Language:English
Published: 2010
Online Access:http://discol.umk.edu.my/id/eprint/9037/1/preliminary%20study%20on%20leech%20crude%20extract%20as%20an%20anti-coagulant.pdf
http://discol.umk.edu.my/id/eprint/9037/
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spelling my.umk.eprints.90372022-05-23T12:26:47Z http://discol.umk.edu.my/id/eprint/9037/ Preliminary study on leech crude extract as an anti-coagulant agent Zulhisyam Abdul Kari @ Abdullah Ahmad Anwar Mohamed Ismail Ibrahim Che Omar Hirudin is the generic name for a family of closely related homologous peptides that are all found in the cranial salivary glands of medicinal leech (Wallis, 1996). In 1984, the blood-thinning agent (anticoagulant) hirudin was isolated for the first time from leech salivary glands (Weinnberg, 1994). Today, hirudin is the most specific and active of known thrombin inhibitors not influencing on other peptidases (Hofsteenge and Stone, 1987). Hirudinea sp. (Buffalo Leech) was used for the isolation of hirudin for anti-coagulant application. The leech crude extract was prepared by using conventional method and tested to fresh rabbit blood. The time of blood clotting was recorded by using a drop method (Prakasam et. al., 2005). Three replicates were applied for each treatment. The results showed that the rabbit blood treated with leech crude extract gave the longest time of blood to clot with average and standard deviation values of 15.33±0.6 minutes when compared to treatment with distilled water and that on blood only (control) with an average and standard deviation values of 2.83±0.3 and 0.59±0.01 minutes, respectively. These results revealed that crude extract of leeches contain an anticoagulant agent to prevent blood from clotting. The study on leech crude extract will be extended to include purification of hirudin for applications as anti-coagulant agent. 2010 Conference or Workshop Item NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/9037/1/preliminary%20study%20on%20leech%20crude%20extract%20as%20an%20anti-coagulant.pdf Zulhisyam Abdul Kari @ Abdullah and Ahmad Anwar Mohamed Ismail and Ibrahim Che Omar (2010) Preliminary study on leech crude extract as an anti-coagulant agent. In: 9th UMT International Symposium on Sustainability Science and Management (UMTAS), 08-11 May 2010, Permai Inn, Kuala Terengganu.
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Hirudin is the generic name for a family of closely related homologous peptides that are all found in the cranial salivary glands of medicinal leech (Wallis, 1996). In 1984, the blood-thinning agent (anticoagulant) hirudin was isolated for the first time from leech salivary glands (Weinnberg, 1994). Today, hirudin is the most specific and active of known thrombin inhibitors not influencing on other peptidases (Hofsteenge and Stone, 1987). Hirudinea sp. (Buffalo Leech) was used for the isolation of hirudin for anti-coagulant application. The leech crude extract was prepared by using conventional method and tested to fresh rabbit blood. The time of blood clotting was recorded by using a drop method (Prakasam et. al., 2005). Three replicates were applied for each treatment. The results showed that the rabbit blood treated with leech crude extract gave the longest time of blood to clot with average and standard deviation values of 15.33±0.6 minutes when compared to treatment with distilled water and that on blood only (control) with an average and standard deviation values of 2.83±0.3 and 0.59±0.01 minutes, respectively. These results revealed that crude extract of leeches contain an anticoagulant agent to prevent blood from clotting. The study on leech crude extract will be extended to include purification of hirudin for applications as anti-coagulant agent.
format Conference or Workshop Item
author Zulhisyam Abdul Kari @ Abdullah
Ahmad Anwar Mohamed Ismail
Ibrahim Che Omar
spellingShingle Zulhisyam Abdul Kari @ Abdullah
Ahmad Anwar Mohamed Ismail
Ibrahim Che Omar
Preliminary study on leech crude extract as an anti-coagulant agent
author_facet Zulhisyam Abdul Kari @ Abdullah
Ahmad Anwar Mohamed Ismail
Ibrahim Che Omar
author_sort Zulhisyam Abdul Kari @ Abdullah
title Preliminary study on leech crude extract as an anti-coagulant agent
title_short Preliminary study on leech crude extract as an anti-coagulant agent
title_full Preliminary study on leech crude extract as an anti-coagulant agent
title_fullStr Preliminary study on leech crude extract as an anti-coagulant agent
title_full_unstemmed Preliminary study on leech crude extract as an anti-coagulant agent
title_sort preliminary study on leech crude extract as an anti-coagulant agent
publishDate 2010
url http://discol.umk.edu.my/id/eprint/9037/1/preliminary%20study%20on%20leech%20crude%20extract%20as%20an%20anti-coagulant.pdf
http://discol.umk.edu.my/id/eprint/9037/
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score 13.211869

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