An improved technique for the rapid chemical characterisation of bacterial terpene cyclases
A derivative of the pET28c(+) expression vector was constructed. It contains a yeast replication system (2μ origin of replication) and a yeast selectable marker (URA3), and can be used for gene cloning in yeast by efficient homologous recombination, and for heterologous expression in E. coli. The ve...
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Main Authors: | Jeroen S. Dickschat, Khomaizon A. K. Pahirulzaman, Patrick Rabe, Tim A. Klapschinski |
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Format: | Non-Indexed Article |
Published: |
2014
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Online Access: | http://discol.umk.edu.my/id/eprint/8102/ http://onlinelibrary.wiley.com/doi/10.1002/cbic.201300763/abstract;jsessionid=410C17355B17CC08D35F227893B84109.f01t01?userIsAuthenticated=false&deniedAccessCustomisedMessage= |
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