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Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant

Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, di...

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Main Authors: Ranajit Kumar Shaha, Nitai Roy, G.Talukdar
Format: Non-Indexed Article
Language:English
Published: Universiti Malaysia Kelantan 2013
Online Access:http://discol.umk.edu.my/id/eprint/7843/1/3.pdf
http://discol.umk.edu.my/id/eprint/7843/
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spelling my.umk.eprints.78432022-05-23T15:13:37Z http://discol.umk.edu.my/id/eprint/7843/ Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant Ranajit Kumar Shaha Nitai Roy G.Talukdar Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, dialysis, gel filtrations and DEAE-Cellulose chromatography and the protein was designated as LFPP (Litchi flowers pollen protein). Gel filtration of the purified pollen protein gives two main peaks. The major peak gives four bands on SDS-PAGE. The enzyme (pectate lyase) proteins after gel filtration again re-purified by Ion exchange chromatography, a single band in the protein profile of LFPP, (M.W. 28kDa) was the major allergenic component of Litchi chinensis (Litchi) flower pollen. The homogeneity and the molecular weight of the protein were estimated by SDS-PAGE, and Gel filtration was 28kDa. The allergenic protein was identified by skin prick tests and showed the pectate lyase (Pel) activity. Skin-prick tests also revealed highest degree of sensitivity to the Nawabgang sample giving positive response in 80% of the patients. Skin reactivity ranged between 1+ and 3+. Universiti Malaysia Kelantan 2013 Non-Indexed Article NonPeerReviewed text en http://discol.umk.edu.my/id/eprint/7843/1/3.pdf Ranajit Kumar Shaha and Nitai Roy and G.Talukdar (2013) Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant. Journal of Tropical Resources and Sustainable Sciences, 1 (1). pp. 25-35. ISSN 2289-3946
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
language English
description Pollen of Litchi chinensis (Litchi) is a major aeroallergen of Bangladesh. Pollen of this fruits plant was collected from full bloomed flower growing in different places of Rajshahi in Bangladesh. Pollen protein was extracted and partial purified by means of long-term PBS extraction, salting out, dialysis, gel filtrations and DEAE-Cellulose chromatography and the protein was designated as LFPP (Litchi flowers pollen protein). Gel filtration of the purified pollen protein gives two main peaks. The major peak gives four bands on SDS-PAGE. The enzyme (pectate lyase) proteins after gel filtration again re-purified by Ion exchange chromatography, a single band in the protein profile of LFPP, (M.W. 28kDa) was the major allergenic component of Litchi chinensis (Litchi) flower pollen. The homogeneity and the molecular weight of the protein were estimated by SDS-PAGE, and Gel filtration was 28kDa. The allergenic protein was identified by skin prick tests and showed the pectate lyase (Pel) activity. Skin-prick tests also revealed highest degree of sensitivity to the Nawabgang sample giving positive response in 80% of the patients. Skin reactivity ranged between 1+ and 3+.
format Non-Indexed Article
author Ranajit Kumar Shaha
Nitai Roy
G.Talukdar
spellingShingle Ranajit Kumar Shaha
Nitai Roy
G.Talukdar
Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
author_facet Ranajit Kumar Shaha
Nitai Roy
G.Talukdar
author_sort Ranajit Kumar Shaha
title Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
title_short Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
title_full Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
title_fullStr Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
title_full_unstemmed Characterization and sensitivity test of the allergenic pollen proteins from Litchi Chimensis Plant
title_sort characterization and sensitivity test of the allergenic pollen proteins from litchi chimensis plant
publisher Universiti Malaysia Kelantan
publishDate 2013
url http://discol.umk.edu.my/id/eprint/7843/1/3.pdf
http://discol.umk.edu.my/id/eprint/7843/
_version_ 1763303898798358528
score 13.211869

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