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Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast

This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dulbecc...

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Main Authors: Mohd Azam Khan Goriman Khan, Fauziah O.
Format: Non-Indexed Article
Published: 2010
Online Access:http://discol.umk.edu.my/id/eprint/7781/
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id my.umk.eprints.7781
record_format eprints
spelling my.umk.eprints.77812022-05-23T10:22:28Z http://discol.umk.edu.my/id/eprint/7781/ Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast Mohd Azam Khan Goriman Khan Fauziah O. This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37oC in 5% CO2. The cell lines were treated with gentamicin-coated HA and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. After 72 hours treatment, the gentamicin-coated HA was processed for viewing the morphological changes by using scanning electron microscopy. The MTT assay results indicated that varying concentrations of gentamicin coated hydroxyapatite do not significantly affect viability of osteoblast. The osteoblast-like cells attached and spread well on the surface of hydroxyapatite and grew into the pores. In conclusion, the gentamicin-coated HA is biocompatible towards osteoblast. 2010-10 Non-Indexed Article NonPeerReviewed Mohd Azam Khan Goriman Khan and Fauziah O. (2010) Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast.
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This study was carried out to compare the surface morphological changes and cell-to-cell attachment with different concentrations of gentamicin-coated hydroxyapatite (HA) on the osteoblast cell lines. Osteoblast cell lines were cultured and maintained in complete medium, 1:1 HAM’s F12 Medium Dulbecco's modified Eagle's medium without phenol red (DMEM) and incubated at 37oC in 5% CO2. The cell lines were treated with gentamicin-coated HA and undergone the 3-(4,5-di-methylthiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) assay. After 72 hours treatment, the gentamicin-coated HA was processed for viewing the morphological changes by using scanning electron microscopy. The MTT assay results indicated that varying concentrations of gentamicin coated hydroxyapatite do not significantly affect viability of osteoblast. The osteoblast-like cells attached and spread well on the surface of hydroxyapatite and grew into the pores. In conclusion, the gentamicin-coated HA is biocompatible towards osteoblast.
format Non-Indexed Article
author Mohd Azam Khan Goriman Khan
Fauziah O.
spellingShingle Mohd Azam Khan Goriman Khan
Fauziah O.
Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
author_facet Mohd Azam Khan Goriman Khan
Fauziah O.
author_sort Mohd Azam Khan Goriman Khan
title Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
title_short Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
title_full Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
title_fullStr Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
title_full_unstemmed Biocompatibility of gentamicin-coated hydroxyapatite (Ha) with osteoblast
title_sort biocompatibility of gentamicin-coated hydroxyapatite (ha) with osteoblast
publishDate 2010
url http://discol.umk.edu.my/id/eprint/7781/
_version_ 1763303889863442432
score 13.211869

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