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Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation

A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation...

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Main Authors: Syarifah Ab Rashid, Darah Ibrahim, Ibrahim Che Omar
Format: Non-Indexed Article
Published: 2011
Online Access:http://discol.umk.edu.my/id/eprint/7645/
http://www.ispub.com/journal/the-internet-journal-of-microbiology/volume-9-number-1/utilization-of-palm-kernel-cake-for-the-production-of-mannanase-by-an-indigenous-filamentous-fungus-aspergillus-niger-usm-f4-under-solid-substrate-fermentation.html
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spelling my.umk.eprints.76452022-05-23T10:21:27Z http://discol.umk.edu.my/id/eprint/7645/ Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation Syarifah Ab Rashid Darah Ibrahim Ibrahim Che Omar A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30ºC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions. 2011 Non-Indexed Article NonPeerReviewed Syarifah Ab Rashid and Darah Ibrahim and Ibrahim Che Omar (2011) Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation. The Internet Journal of Microbiology, 9 (1). pp. 1-19. ISSN 1937-8289 http://www.ispub.com/journal/the-internet-journal-of-microbiology/volume-9-number-1/utilization-of-palm-kernel-cake-for-the-production-of-mannanase-by-an-indigenous-filamentous-fungus-aspergillus-niger-usm-f4-under-solid-substrate-fermentation.html
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description A mannanase yielding indigenous fungal culture Aspergillus niger USM F4 which was isolated from decayed palm kernel cake (PKC) collected from a local oil palm processed plant was used in this study. The fungal culture produced high yields of extracellular mannanase under solid-substrate fermentation (SSF) using PKC as a substrate. The results obtained from this study revealed that after five days of cultivation period, a maximum extracellular mannanase activity was 433.84 U/ g substrate and 0.96 mg glucosamine/g substrate of fungal growth. The optimized cultural conditions and medium compositions were 10 g PKC with particle size of 0.5 mm, moisture content of 80% (v/w), inoculums size of 1x107 spores/ml, incubation temperature of 30ºC, static (without mixing), 2% (w/w) of molasses and 4% (w/w) of ammonium nitrate. There was 53.68% increment of mannanase production after optimization compared to before optimization of cultural conditions and medium compositions.
format Non-Indexed Article
author Syarifah Ab Rashid
Darah Ibrahim
Ibrahim Che Omar
spellingShingle Syarifah Ab Rashid
Darah Ibrahim
Ibrahim Che Omar
Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
author_facet Syarifah Ab Rashid
Darah Ibrahim
Ibrahim Che Omar
author_sort Syarifah Ab Rashid
title Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
title_short Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
title_full Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
title_fullStr Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
title_full_unstemmed Utilization of Palm Kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger USM F4 under solid substrate fermentation
title_sort utilization of palm kernel cake for the production of mannanase by an indigenous filamentous fungus, aspergillus niger usm f4 under solid substrate fermentation
publishDate 2011
url http://discol.umk.edu.my/id/eprint/7645/
http://www.ispub.com/journal/the-internet-journal-of-microbiology/volume-9-number-1/utilization-of-palm-kernel-cake-for-the-production-of-mannanase-by-an-indigenous-filamentous-fungus-aspergillus-niger-usm-f4-under-solid-substrate-fermentation.html
_version_ 1763303870832836608
score 13.211869

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