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Identification of new genomospecies in the mycobacterium terrae complex

Members of the Mycobacterium terrae complex are slow-growing, non-chromogenic acidfast bacilli found in the natural environment and occasionally in clinical material. These genetically closely-related members are difficult to differentiate by conventional phenotypic and molecular tests. In this pape...

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Main Authors: Yun Fong Ngeow, Yan Ling Wong, Joon Liang Tan, Kar Wai Hong, Hien Fuh Ng, Bee Lee Ong, Kok Gan Chan
Format: Indexed Article
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/7511/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382200/pdf/pone.0120789.pdf
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spelling my.umk.eprints.75112022-05-23T10:00:41Z http://discol.umk.edu.my/id/eprint/7511/ Identification of new genomospecies in the mycobacterium terrae complex Yun Fong Ngeow Yan Ling Wong Joon Liang Tan Kar Wai Hong Hien Fuh Ng Bee Lee Ong Kok Gan Chan Members of the Mycobacterium terrae complex are slow-growing, non-chromogenic acidfast bacilli found in the natural environment and occasionally in clinical material. These genetically closely-related members are difficult to differentiate by conventional phenotypic and molecular tests. In this paper we describe the use of whole genome data for the identification of four strains genetically similar to Mycobacterium sp. JDM601, a newly identified member of the M. terrae complex. Phylogenetic information from the alignment of genomewide orthologous genes and single nucleotide polymorphisms show consistent clustering of the four strains together with M. sp. JDM601 into a distinct clade separate from other rapid and slow growing mycobacterial species. More detailed inter-strain comparisons using average nucleotide identity, tetra-nucleotide frequencies and analysis of synteny indicate that our strains are closely related to but not of the same species as M. sp. JDM601. Besides the 16S rRNA signature described previously for the M. terrae complex, five more hypothetical proteins were found that are potentially useful for the rapid identification of mycobacterial species belonging to the M. terrae complex. This paper illustrates the versatile utilization of whole genome data for the delineation of new bacterial species and introduces four new genomospecies to add to current members in the M. terrae complex. 2015 Indexed Article NonPeerReviewed Yun Fong Ngeow and Yan Ling Wong and Joon Liang Tan and Kar Wai Hong and Hien Fuh Ng and Bee Lee Ong and Kok Gan Chan (2015) Identification of new genomospecies in the mycobacterium terrae complex. Plos One, 10 (4). pp. 1-16. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382200/pdf/pone.0120789.pdf
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Members of the Mycobacterium terrae complex are slow-growing, non-chromogenic acidfast bacilli found in the natural environment and occasionally in clinical material. These genetically closely-related members are difficult to differentiate by conventional phenotypic and molecular tests. In this paper we describe the use of whole genome data for the identification of four strains genetically similar to Mycobacterium sp. JDM601, a newly identified member of the M. terrae complex. Phylogenetic information from the alignment of genomewide orthologous genes and single nucleotide polymorphisms show consistent clustering of the four strains together with M. sp. JDM601 into a distinct clade separate from other rapid and slow growing mycobacterial species. More detailed inter-strain comparisons using average nucleotide identity, tetra-nucleotide frequencies and analysis of synteny indicate that our strains are closely related to but not of the same species as M. sp. JDM601. Besides the 16S rRNA signature described previously for the M. terrae complex, five more hypothetical proteins were found that are potentially useful for the rapid identification of mycobacterial species belonging to the M. terrae complex. This paper illustrates the versatile utilization of whole genome data for the delineation of new bacterial species and introduces four new genomospecies to add to current members in the M. terrae complex.
format Indexed Article
author Yun Fong Ngeow
Yan Ling Wong
Joon Liang Tan
Kar Wai Hong
Hien Fuh Ng
Bee Lee Ong
Kok Gan Chan
spellingShingle Yun Fong Ngeow
Yan Ling Wong
Joon Liang Tan
Kar Wai Hong
Hien Fuh Ng
Bee Lee Ong
Kok Gan Chan
Identification of new genomospecies in the mycobacterium terrae complex
author_facet Yun Fong Ngeow
Yan Ling Wong
Joon Liang Tan
Kar Wai Hong
Hien Fuh Ng
Bee Lee Ong
Kok Gan Chan
author_sort Yun Fong Ngeow
title Identification of new genomospecies in the mycobacterium terrae complex
title_short Identification of new genomospecies in the mycobacterium terrae complex
title_full Identification of new genomospecies in the mycobacterium terrae complex
title_fullStr Identification of new genomospecies in the mycobacterium terrae complex
title_full_unstemmed Identification of new genomospecies in the mycobacterium terrae complex
title_sort identification of new genomospecies in the mycobacterium terrae complex
publishDate 2015
url http://discol.umk.edu.my/id/eprint/7511/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4382200/pdf/pone.0120789.pdf
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score 13.211869

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