Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts
Ethnopharmacological relevance: Local communities in Malaysia consume Pereskia bleo Kunth. (Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes, hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitaliz- ing the...
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my.umk.eprints.75022022-05-23T10:00:37Z http://discol.umk.edu.my/id/eprint/7502/ Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts Ikarastika Rahayu Abdul Wahab Carolina Carvalho Guilhon Patricia Dias Fernandes Fabio Boylan Ethnopharmacological relevance: Local communities in Malaysia consume Pereskia bleo Kunth. (Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes, hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitaliz- ing the body. Aim of the study: To evaluate anti-nociceptive activity of the extracts and vitexin, isolated for the first time in this species, in two analgesic models; formalin-induced licking and acetic acid-induced abdominal writhing. Materials and methods: Three and a half kilos of P. bleo leaves were extracted using Soxhlet apparatus with ethanol for 72 h. The crude ethanol extract was treated with activated charcoal overnight and subjected to a liquid–liquid partition yielding hexane, dichloromethane, ethyl acetate and butanol extracts. All extracts, including the crude ethanol and vitexin isolated from the ethyl acetate partition were tested for peripheral anti-nociceptive activity using formalin test and acetic acid-induced abdominal writhing, besides having their acute toxicity assays performed. Results: The phytochemical analyses resulted in the isolation of vitexin (1), b-sitosterol glucoside (2) and b-sitosterol (3) isolated from the ethyl acetate, dichloromethane and hexane extracts, respectively. This is the first time vitexin and b-sitosterol glucoside are isolated from this species. The anti-nociceptive activities for all extracts were only moderate. Vitexin, which was isolated from the ethyl acetate extract did not show any activity in all models tested when used alone at the same concentration as it appears in the extract. Conclusion: This study showed that all the extracts possess moderate anti-nociceptive activity. Vitexin is not the compound responsible for the anti-nociceptive effect in the ethyl acetate extract. Further investigations are needed to identify the compound(s) that might be responsible for the anti- nociceptive activity in this plant. Elsevier 2012 Indexed Article NonPeerReviewed Ikarastika Rahayu Abdul Wahab and Carolina Carvalho Guilhon and Patricia Dias Fernandes and Fabio Boylan (2012) Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts. Journal of Ethnopharmacology, 144. pp. 741-746. ISSN 0378-8741 |
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| description |
Ethnopharmacological relevance: Local communities in Malaysia consume Pereskia bleo Kunth.
(Cactaceae) leaves as raw vegetables or as a concoction and drink as a tea to treat diabetes,
hypertension, rheumatism, cancer-related diseases, inflammation, gastric pain, ulcers, and for revitaliz-
ing the body.
Aim of the study: To evaluate anti-nociceptive activity of the extracts and vitexin, isolated for the first
time in this species, in two analgesic models; formalin-induced licking and acetic acid-induced
abdominal writhing.
Materials and methods: Three and a half kilos of P. bleo leaves were extracted using Soxhlet apparatus
with ethanol for 72 h. The crude ethanol extract was treated with activated charcoal overnight and
subjected to a liquid–liquid partition yielding hexane, dichloromethane, ethyl acetate and butanol
extracts. All extracts, including the crude ethanol and vitexin isolated from the ethyl acetate partition
were tested for peripheral anti-nociceptive activity using formalin test and acetic acid-induced
abdominal writhing, besides having their acute toxicity assays performed.
Results: The phytochemical analyses resulted in the isolation of vitexin (1), b-sitosterol glucoside
(2) and b-sitosterol (3) isolated from the ethyl acetate, dichloromethane and hexane extracts,
respectively. This is the first time vitexin and b-sitosterol glucoside are isolated from this species.
The anti-nociceptive activities for all extracts were only moderate. Vitexin, which was isolated from the
ethyl acetate extract did not show any activity in all models tested when used alone at the same
concentration as it appears in the extract.
Conclusion: This study showed that all the extracts possess moderate anti-nociceptive activity. Vitexin
is not the compound responsible for the anti-nociceptive effect in the ethyl acetate extract. Further
investigations are needed to identify the compound(s) that might be responsible for the anti-
nociceptive activity in this plant. |
| format |
Indexed Article |
| author |
Ikarastika Rahayu Abdul Wahab Carolina Carvalho Guilhon Patricia Dias Fernandes Fabio Boylan |
| spellingShingle |
Ikarastika Rahayu Abdul Wahab Carolina Carvalho Guilhon Patricia Dias Fernandes Fabio Boylan Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| author_facet |
Ikarastika Rahayu Abdul Wahab Carolina Carvalho Guilhon Patricia Dias Fernandes Fabio Boylan |
| author_sort |
Ikarastika Rahayu Abdul Wahab |
| title |
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| title_short |
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| title_full |
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| title_fullStr |
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| title_full_unstemmed |
Anti-nociceptive activity of Pereskia bleo Kunth. (Cactaceae) leaves extracts |
| title_sort |
anti-nociceptive activity of pereskia bleo kunth. (cactaceae) leaves extracts |
| publisher |
Elsevier |
| publishDate |
2012 |
| url |
http://discol.umk.edu.my/id/eprint/7502/ |
| _version_ |
1763303850671865856 |
| score |
13.211869 |