Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants
Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneid...
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| Format: | Undergraduate Final Project Report |
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2016
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| Online Access: | http://discol.umk.edu.my/id/eprint/6622/ |
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my.umk.eprints.66222022-05-23T08:48:21Z http://discol.umk.edu.my/id/eprint/6622/ Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants Umi Nor Zarina Othman Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneidensis MR-1 respiratory mutants unable to reduce Nitrate (N0₃) as anaerobic electron acceptor. S.oneidensis nitrate-reductant deficient mutants screening technique was based on the observation that wild-type S. oneidensis produced a red precipitate on its colony surface during screening test, while colonies arising from mutagenized cells did not changed the color at all. The cell density of mutagenized cells 0.2396 (±.152) is lower compared to wild type control 0.3318 (±.576). This report describes the design of rapid screening technique and its application to identify mutants in a metal-reducing member of the genus Shewanella. 2016 Undergraduate Final Project Report NonPeerReviewed Umi Nor Zarina Othman (2016) Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted) |
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| description |
Shewanella oneidensisis is an important model organism for bioremediation studies and electrical generation because of its diverse respiratory capabilities, conferred in part by multicomponent, branched electron transport systems. A rapid mutant screening technique was developed to identify S. oneidensis MR-1 respiratory mutants unable to reduce Nitrate (N0₃) as anaerobic electron acceptor. S.oneidensis nitrate-reductant deficient mutants screening technique was based on the observation that wild-type S. oneidensis produced a red precipitate on its colony surface during screening test, while colonies arising from mutagenized cells did not changed the color at all. The cell density of mutagenized cells 0.2396 (±.152) is lower compared to wild type control 0.3318 (±.576). This report describes the design of rapid screening technique and its application to identify mutants in a metal-reducing member of the genus Shewanella. |
| format |
Undergraduate Final Project Report |
| author |
Umi Nor Zarina Othman |
| spellingShingle |
Umi Nor Zarina Othman Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| author_facet |
Umi Nor Zarina Othman |
| author_sort |
Umi Nor Zarina Othman |
| title |
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| title_short |
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| title_full |
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| title_fullStr |
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| title_full_unstemmed |
Design of a rapid screening technique for identification of Shewanella Oneidensis nitrate-reductant deficient mutants |
| title_sort |
design of a rapid screening technique for identification of shewanella oneidensis nitrate-reductant deficient mutants |
| publishDate |
2016 |
| url |
http://discol.umk.edu.my/id/eprint/6622/ |
| _version_ |
1763303723511054336 |
| score |
13.211869 |