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Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants

Introduction : Microbial metal reduction forms the basis of alternate bioremediationstrategies for reductive precipitation and immobilization of toxic metals such as Fe(III). Objectives : A rapid screening techniques were developed to identify Shewanella oneidensis MR-1 respiratory mutants unable to...

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Main Author: Nor Mazalfala Aridzi
Format: Undergraduate Final Project Report
Published: 2016
Online Access:http://discol.umk.edu.my/id/eprint/6585/
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spelling my.umk.eprints.65852022-05-23T08:48:07Z http://discol.umk.edu.my/id/eprint/6585/ Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants Nor Mazalfala Aridzi Introduction : Microbial metal reduction forms the basis of alternate bioremediationstrategies for reductive precipitation and immobilization of toxic metals such as Fe(III). Objectives : A rapid screening techniques were developed to identify Shewanella oneidensis MR-1 respiratory mutants unable to reduce Fe(III) as anaerobic electron acceptor. Method : The Fe(III) reduction-deficient mutant screening technique was based on the observation that wild-type Shewanella Oneidensis produced a dark blue precipitate on its colony surface during growth on Fe(III) while colony arising from mutagenesis did not. Result : Three of the mutant strains were unable to produce the dark blue precipitate after tested for anaerobic growth in a 96-well plates. The result present study shows that Shewanella Oneidensis iron-reduction deficient mutants was generated and yet retained the ability of reducing Fe(III) to Fe(II). Conclusion : This study describes the development of a mutant screening technique and its application to identify a set of mutants in a metal-reducing member of the genus Shewanella in anaerobic condition which is use in a bioremediation process. 2016 Undergraduate Final Project Report NonPeerReviewed Nor Mazalfala Aridzi (2016) Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Introduction : Microbial metal reduction forms the basis of alternate bioremediationstrategies for reductive precipitation and immobilization of toxic metals such as Fe(III). Objectives : A rapid screening techniques were developed to identify Shewanella oneidensis MR-1 respiratory mutants unable to reduce Fe(III) as anaerobic electron acceptor. Method : The Fe(III) reduction-deficient mutant screening technique was based on the observation that wild-type Shewanella Oneidensis produced a dark blue precipitate on its colony surface during growth on Fe(III) while colony arising from mutagenesis did not. Result : Three of the mutant strains were unable to produce the dark blue precipitate after tested for anaerobic growth in a 96-well plates. The result present study shows that Shewanella Oneidensis iron-reduction deficient mutants was generated and yet retained the ability of reducing Fe(III) to Fe(II). Conclusion : This study describes the development of a mutant screening technique and its application to identify a set of mutants in a metal-reducing member of the genus Shewanella in anaerobic condition which is use in a bioremediation process.
format Undergraduate Final Project Report
author Nor Mazalfala Aridzi
spellingShingle Nor Mazalfala Aridzi
Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
author_facet Nor Mazalfala Aridzi
author_sort Nor Mazalfala Aridzi
title Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
title_short Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
title_full Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
title_fullStr Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
title_full_unstemmed Design of rapid screening technique for identification of Shewanella oneidensis iron-reduction deficient mutants
title_sort design of rapid screening technique for identification of shewanella oneidensis iron-reduction deficient mutants
publishDate 2016
url http://discol.umk.edu.my/id/eprint/6585/
_version_ 1763303718127665152
score 13.211869

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