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Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments

Utilitarian strains/consortia for experience ammonium oxidation coupled to iron reduction divergence, termed as Feammox incorporate unique bacterial strains that are not been reported previously. In the present study, anaerobic oxidation of ammonium to nitrite has been observed in wetland soils unde...

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Main Author: Noor Ezanna Khamidun
Format: Undergraduate Final Project Report
Published: 2016
Online Access:http://discol.umk.edu.my/id/eprint/6580/
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spelling my.umk.eprints.65802022-05-23T08:48:05Z http://discol.umk.edu.my/id/eprint/6580/ Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments Noor Ezanna Khamidun Utilitarian strains/consortia for experience ammonium oxidation coupled to iron reduction divergence, termed as Feammox incorporate unique bacterial strains that are not been reported previously. In the present study, anaerobic oxidation of ammonium to nitrite has been observed in wetland soils under iron-reducing conditions. In exploring the dynamics of iron and nitrogen cycling in tropical forest soils and paddy fields sediments, we had observed a redox reaction that has not been previously described. To dictate whether ammonium oxidation under iron reducing conditions, each ammonium sulphate concentration was manipulated ranging from 1mM, 5mM and 20mM and functioned as electron donor while FeOOH as final terminal of electron acceptor in the certain cytochrome of electron transport chain. Development of enrichment culture revealed that Fe (Ill) are reduced to Fe (ll) by employed color indicator appeared oranges to dark. During incubations of soil slurries under anaerobic conditions, we measured an unexpected total concentration ferrous iron, Fe (ll) via ferrozine method. From the manipulation the source of electron, we found that Fe (ll) productions by newly isolated EF/D1 strains after 20-30 days anaerobic incubation in serum bottle containing Westlake supplemented with 20mM HFO and 1mM NH₄⁺ showed the highest concentration amongst all. This indirect evidence, we conclude that under anoxic and limited oxygen sources conditions, there is a biological process that utilizes ferric iron Fe (III) as an electron acceptor while oxidizing ammonium (NH₄⁺), termed as Feammox. We believed that Feammox could trigger nitrogen losses in ecosystems rich in poorly crystalline iron minerals, with low or fluctuating redox reactions. 2016 Undergraduate Final Project Report NonPeerReviewed Noor Ezanna Khamidun (2016) Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Utilitarian strains/consortia for experience ammonium oxidation coupled to iron reduction divergence, termed as Feammox incorporate unique bacterial strains that are not been reported previously. In the present study, anaerobic oxidation of ammonium to nitrite has been observed in wetland soils under iron-reducing conditions. In exploring the dynamics of iron and nitrogen cycling in tropical forest soils and paddy fields sediments, we had observed a redox reaction that has not been previously described. To dictate whether ammonium oxidation under iron reducing conditions, each ammonium sulphate concentration was manipulated ranging from 1mM, 5mM and 20mM and functioned as electron donor while FeOOH as final terminal of electron acceptor in the certain cytochrome of electron transport chain. Development of enrichment culture revealed that Fe (Ill) are reduced to Fe (ll) by employed color indicator appeared oranges to dark. During incubations of soil slurries under anaerobic conditions, we measured an unexpected total concentration ferrous iron, Fe (ll) via ferrozine method. From the manipulation the source of electron, we found that Fe (ll) productions by newly isolated EF/D1 strains after 20-30 days anaerobic incubation in serum bottle containing Westlake supplemented with 20mM HFO and 1mM NH₄⁺ showed the highest concentration amongst all. This indirect evidence, we conclude that under anoxic and limited oxygen sources conditions, there is a biological process that utilizes ferric iron Fe (III) as an electron acceptor while oxidizing ammonium (NH₄⁺), termed as Feammox. We believed that Feammox could trigger nitrogen losses in ecosystems rich in poorly crystalline iron minerals, with low or fluctuating redox reactions.
format Undergraduate Final Project Report
author Noor Ezanna Khamidun
spellingShingle Noor Ezanna Khamidun
Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
author_facet Noor Ezanna Khamidun
author_sort Noor Ezanna Khamidun
title Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
title_short Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
title_full Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
title_fullStr Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
title_full_unstemmed Enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
title_sort enrichment of iron-dependent anaerobic ammonia oxidizer microbial communities from tropical forest soil and paddy field sediments
publishDate 2016
url http://discol.umk.edu.my/id/eprint/6580/
_version_ 1763303717398904832
score 13.211869

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