Cover Image

Screening of exopolysaccharide producing bacteria strains

This thesis presents a novel improved screening approach in discovering excessive exopolysaccharide producing bacteria strains. Patch isolation method is a rapid screening technique to reveal exopolysaccharide producing bacteria strains within a short period of time. In the present studies, aniline...

Full description

Saved in:
Bibliographic Details
Main Author: Chee, Mee Teng
Format: Undergraduate Final Project Report
Published: 2016
Online Access:http://discol.umk.edu.my/id/eprint/6537/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.6537
record_format eprints
spelling my.umk.eprints.65372022-05-23T08:47:49Z http://discol.umk.edu.my/id/eprint/6537/ Screening of exopolysaccharide producing bacteria strains Chee, Mee Teng This thesis presents a novel improved screening approach in discovering excessive exopolysaccharide producing bacteria strains. Patch isolation method is a rapid screening technique to reveal exopolysaccharide producing bacteria strains within a short period of time. In the present studies, aniline blue is used as the biological indicator to identify the production activity of exopolysaccharides. Presence of blue colour intensity indicates the production rate of exopolysaccharides. Excessive exopolysaccharide producing bacteria strains can be found in soil microbial communities. Soil bacteria requires carbon source as metabolic needs to produce exopolysaccharides. Table sugar used as the lower cost sugar substrate to grow the isolated bacteria. The rate of carbon source required to produce exopolysaccharides is depends on the types and behaviours of bacterial isolates. To determine whether the carbon source affect the production activity of exopolysaccharides, 1%, 5% and 20% sugar concentration were used as the parameter for cultivation conditions of soil bacterial isolates. The screening bacterial isolates showing positive responses were further to production of exopolysaccharides and the resulting exopolysaccharides were extracted. The extracted exopolysaccharides displayed different characteristics compare to the positive control, Agrobacterium sp. ATCC 31749 and yet to be explored more on the functional properties. 2016 Undergraduate Final Project Report NonPeerReviewed Chee, Mee Teng (2016) Screening of exopolysaccharide producing bacteria strains. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This thesis presents a novel improved screening approach in discovering excessive exopolysaccharide producing bacteria strains. Patch isolation method is a rapid screening technique to reveal exopolysaccharide producing bacteria strains within a short period of time. In the present studies, aniline blue is used as the biological indicator to identify the production activity of exopolysaccharides. Presence of blue colour intensity indicates the production rate of exopolysaccharides. Excessive exopolysaccharide producing bacteria strains can be found in soil microbial communities. Soil bacteria requires carbon source as metabolic needs to produce exopolysaccharides. Table sugar used as the lower cost sugar substrate to grow the isolated bacteria. The rate of carbon source required to produce exopolysaccharides is depends on the types and behaviours of bacterial isolates. To determine whether the carbon source affect the production activity of exopolysaccharides, 1%, 5% and 20% sugar concentration were used as the parameter for cultivation conditions of soil bacterial isolates. The screening bacterial isolates showing positive responses were further to production of exopolysaccharides and the resulting exopolysaccharides were extracted. The extracted exopolysaccharides displayed different characteristics compare to the positive control, Agrobacterium sp. ATCC 31749 and yet to be explored more on the functional properties.
format Undergraduate Final Project Report
author Chee, Mee Teng
spellingShingle Chee, Mee Teng
Screening of exopolysaccharide producing bacteria strains
author_facet Chee, Mee Teng
author_sort Chee, Mee Teng
title Screening of exopolysaccharide producing bacteria strains
title_short Screening of exopolysaccharide producing bacteria strains
title_full Screening of exopolysaccharide producing bacteria strains
title_fullStr Screening of exopolysaccharide producing bacteria strains
title_full_unstemmed Screening of exopolysaccharide producing bacteria strains
title_sort screening of exopolysaccharide producing bacteria strains
publishDate 2016
url http://discol.umk.edu.my/id/eprint/6537/
_version_ 1763303711172460544
score 13.211869

Similar Items