Study on enzymatic detergent formulation containing thermostable protease

This study involves protease production from E. coli BL21 (DE3) pLysS harbouring 50a protease gene, formulation of liquid detergent and its washing performance analysis. Three types of detergents labeled Detergent A, Detergent B and Detergent C having different concentration of anionic surfactants (...

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Main Author: Basyirah Radzuan
Format: Undergraduate Final Project Report
Published: 2016
Online Access:http://discol.umk.edu.my/id/eprint/6533/
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spelling my.umk.eprints.65332022-05-23T08:47:48Z http://discol.umk.edu.my/id/eprint/6533/ Study on enzymatic detergent formulation containing thermostable protease Basyirah Radzuan This study involves protease production from E. coli BL21 (DE3) pLysS harbouring 50a protease gene, formulation of liquid detergent and its washing performance analysis. Three types of detergents labeled Detergent A, Detergent B and Detergent C having different concentration of anionic surfactants (Tween-20) at 3%, 5% and 10% respectively have been formulated through this study. Washing performance test were carried out based on the optimum conditions obtained through previous research at 40 °C, 100 rpm for 1 hour. This experiment had proved that 50a protease showed the most compatibility and stability to Detergent C. it was exposed that among different conditions of washing tested, the combination of 50a protease with detergents exhibited better cleaning action by showing faintness of blood stains. The stains removal evaluation on different condition of washing treatment obtained as followed order: (Detergent supplemented with 50a protease > 50a protease only > Detergent only >: Distilled water only), showed a great potential application of 50a protease in laundry detergents. 2016 Undergraduate Final Project Report NonPeerReviewed Basyirah Radzuan (2016) Study on enzymatic detergent formulation containing thermostable protease. ["eprint_fieldopt_thesis_type_postgraduate" not defined] thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This study involves protease production from E. coli BL21 (DE3) pLysS harbouring 50a protease gene, formulation of liquid detergent and its washing performance analysis. Three types of detergents labeled Detergent A, Detergent B and Detergent C having different concentration of anionic surfactants (Tween-20) at 3%, 5% and 10% respectively have been formulated through this study. Washing performance test were carried out based on the optimum conditions obtained through previous research at 40 °C, 100 rpm for 1 hour. This experiment had proved that 50a protease showed the most compatibility and stability to Detergent C. it was exposed that among different conditions of washing tested, the combination of 50a protease with detergents exhibited better cleaning action by showing faintness of blood stains. The stains removal evaluation on different condition of washing treatment obtained as followed order: (Detergent supplemented with 50a protease > 50a protease only > Detergent only >: Distilled water only), showed a great potential application of 50a protease in laundry detergents.
format Undergraduate Final Project Report
author Basyirah Radzuan
spellingShingle Basyirah Radzuan
Study on enzymatic detergent formulation containing thermostable protease
author_facet Basyirah Radzuan
author_sort Basyirah Radzuan
title Study on enzymatic detergent formulation containing thermostable protease
title_short Study on enzymatic detergent formulation containing thermostable protease
title_full Study on enzymatic detergent formulation containing thermostable protease
title_fullStr Study on enzymatic detergent formulation containing thermostable protease
title_full_unstemmed Study on enzymatic detergent formulation containing thermostable protease
title_sort study on enzymatic detergent formulation containing thermostable protease
publishDate 2016
url http://discol.umk.edu.my/id/eprint/6533/
_version_ 1763303710618812416
score 13.211869