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Study on antioxidant activities and total phenolic content of Leucaena leucocephala species

Plants contain a variety of substances called phytochemicals for example phenolic compounds and antioxidants. Antioxidants are compounds that can delay or inhibit the oxidation of lipids or other molecules by inhibiting the initiation or propagation ofoxidative chain reactions. Phenolic compounds ar...

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Main Author: Nurul Nadia Aminuddin
Format: Undergraduate Final Project Report
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/6327/
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spelling my.umk.eprints.63272022-05-23T08:46:32Z http://discol.umk.edu.my/id/eprint/6327/ Study on antioxidant activities and total phenolic content of Leucaena leucocephala species Nurul Nadia Aminuddin Plants contain a variety of substances called phytochemicals for example phenolic compounds and antioxidants. Antioxidants are compounds that can delay or inhibit the oxidation of lipids or other molecules by inhibiting the initiation or propagation ofoxidative chain reactions. Phenolic compounds are plant secondary metabolites, which play important roles in disease resistance. The antioxidant activity of most of the plant produced is mainly due to the presence of phenolic compounds. Among the phytochemicals. phenolic compounds are the main contributor of antioxidant activity in plant extracts due to their higher value in total phenolic content. The purpose of this study is to determine the amount of total phenolic content present in Leucaena leucocephala species and the relationship between the antioxidant activities and total phenolic content of the Leucaena leucocephala species. Bark and wood of Leucaena leucocephala species were extracted with methanol using Soxhlet Extraction. The total phenolic content was measured using Folin-Ciocalteu method based on standard calibration curve of Gallic acid using. UV-Spectrophotometer. Meanwhile, the free radical capacity was evaluated using DPPH (2, 2- diphenyl-1- picrylhydrazyl) free radical scavenging assay. The present study showed that the bottom bark had the highest total phenolic content followed by middle bark and top wood. High antioxidant activity was shown in bottom bark extracts with EC₅₀ value is 48μg/ml as compared to the other samples. There was a relationship between total phenolic content and antioxidant activities. The results of the bark of Leucaena leucocephala species suggest that these extracts have great source of natural antioxidants. 2015 Undergraduate Final Project Report NonPeerReviewed Nurul Nadia Aminuddin (2015) Study on antioxidant activities and total phenolic content of Leucaena leucocephala species. Undergraduate Final Project Report thesis, Faculty of Earth Science. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Plants contain a variety of substances called phytochemicals for example phenolic compounds and antioxidants. Antioxidants are compounds that can delay or inhibit the oxidation of lipids or other molecules by inhibiting the initiation or propagation ofoxidative chain reactions. Phenolic compounds are plant secondary metabolites, which play important roles in disease resistance. The antioxidant activity of most of the plant produced is mainly due to the presence of phenolic compounds. Among the phytochemicals. phenolic compounds are the main contributor of antioxidant activity in plant extracts due to their higher value in total phenolic content. The purpose of this study is to determine the amount of total phenolic content present in Leucaena leucocephala species and the relationship between the antioxidant activities and total phenolic content of the Leucaena leucocephala species. Bark and wood of Leucaena leucocephala species were extracted with methanol using Soxhlet Extraction. The total phenolic content was measured using Folin-Ciocalteu method based on standard calibration curve of Gallic acid using. UV-Spectrophotometer. Meanwhile, the free radical capacity was evaluated using DPPH (2, 2- diphenyl-1- picrylhydrazyl) free radical scavenging assay. The present study showed that the bottom bark had the highest total phenolic content followed by middle bark and top wood. High antioxidant activity was shown in bottom bark extracts with EC₅₀ value is 48μg/ml as compared to the other samples. There was a relationship between total phenolic content and antioxidant activities. The results of the bark of Leucaena leucocephala species suggest that these extracts have great source of natural antioxidants.
format Undergraduate Final Project Report
author Nurul Nadia Aminuddin
spellingShingle Nurul Nadia Aminuddin
Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
author_facet Nurul Nadia Aminuddin
author_sort Nurul Nadia Aminuddin
title Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
title_short Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
title_full Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
title_fullStr Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
title_full_unstemmed Study on antioxidant activities and total phenolic content of Leucaena leucocephala species
title_sort study on antioxidant activities and total phenolic content of leucaena leucocephala species
publishDate 2015
url http://discol.umk.edu.my/id/eprint/6327/
_version_ 1763303680772145152
score 13.211869

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