Cover Image

Antibacterial mechanism of borreria laevicaulis plant extracts

Outbreaks of emerging infectious diseases following by increase resistance of microorganisms is occur currently at an alarming rate that caused worldwide concern. Hence, there is an urge of developing novel antibiotics in order to combat emerging and re-emerging infectious diseases. To study the ant...

Full description

Saved in:
Bibliographic Details
Main Author: Ng, Kei Vinn
Format: Undergraduate Final Project Report
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/6134/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.6134
record_format eprints
spelling my.umk.eprints.61342022-05-23T08:45:21Z http://discol.umk.edu.my/id/eprint/6134/ Antibacterial mechanism of borreria laevicaulis plant extracts Ng, Kei Vinn Outbreaks of emerging infectious diseases following by increase resistance of microorganisms is occur currently at an alarming rate that caused worldwide concern. Hence, there is an urge of developing novel antibiotics in order to combat emerging and re-emerging infectious diseases. To study the antimicrobial mechanisms of Borreria laevicaulis extracts against gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Salmonella thyphimurium) along with its antibacterial susceptibility test. Extracts of the plants were prepared with solvents of different polarity using soxhlet apparatus. Plants were screened for their antimicrobial activity by using well diffusion assay and minimum inhibitory concentration (MIC) broth dilution method. Bacteria was further tested for antibacterial mechanism that included Scanning Electron Microscope, DNA isolation and analysis and Protein Isolation and analysis. The antibacterial susceptibility test revealed that all of the plant extracts were capable to inhibit the bacteria growth. For antibacterial mechanism, hexane and acetone extract were showing effect on the bacteria cultures as there is disruption on the cell surface. While for DNA isolation and analysis, most of the treated cultures were showing faint fluorescence band compared to untreated cultures. The antibacterial mechanism for protein isolation and analysis was negative as the protein band for all the treated cultures were unaffected and same with the treated cultures. This study proven and supported the use of Borreria laevicaulis which has a broad spectrum of antimicrobial activity in traditional remedies and able to be developed as a potential antimicrobial drugs. 2015 Undergraduate Final Project Report NonPeerReviewed Ng, Kei Vinn (2015) Antibacterial mechanism of borreria laevicaulis plant extracts. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Outbreaks of emerging infectious diseases following by increase resistance of microorganisms is occur currently at an alarming rate that caused worldwide concern. Hence, there is an urge of developing novel antibiotics in order to combat emerging and re-emerging infectious diseases. To study the antimicrobial mechanisms of Borreria laevicaulis extracts against gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Salmonella thyphimurium) along with its antibacterial susceptibility test. Extracts of the plants were prepared with solvents of different polarity using soxhlet apparatus. Plants were screened for their antimicrobial activity by using well diffusion assay and minimum inhibitory concentration (MIC) broth dilution method. Bacteria was further tested for antibacterial mechanism that included Scanning Electron Microscope, DNA isolation and analysis and Protein Isolation and analysis. The antibacterial susceptibility test revealed that all of the plant extracts were capable to inhibit the bacteria growth. For antibacterial mechanism, hexane and acetone extract were showing effect on the bacteria cultures as there is disruption on the cell surface. While for DNA isolation and analysis, most of the treated cultures were showing faint fluorescence band compared to untreated cultures. The antibacterial mechanism for protein isolation and analysis was negative as the protein band for all the treated cultures were unaffected and same with the treated cultures. This study proven and supported the use of Borreria laevicaulis which has a broad spectrum of antimicrobial activity in traditional remedies and able to be developed as a potential antimicrobial drugs.
format Undergraduate Final Project Report
author Ng, Kei Vinn
spellingShingle Ng, Kei Vinn
Antibacterial mechanism of borreria laevicaulis plant extracts
author_facet Ng, Kei Vinn
author_sort Ng, Kei Vinn
title Antibacterial mechanism of borreria laevicaulis plant extracts
title_short Antibacterial mechanism of borreria laevicaulis plant extracts
title_full Antibacterial mechanism of borreria laevicaulis plant extracts
title_fullStr Antibacterial mechanism of borreria laevicaulis plant extracts
title_full_unstemmed Antibacterial mechanism of borreria laevicaulis plant extracts
title_sort antibacterial mechanism of borreria laevicaulis plant extracts
publishDate 2015
url http://discol.umk.edu.my/id/eprint/6134/
_version_ 1763303653144264704
score 13.211869

Similar Items