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Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste

This study is aim to determine production of cellulase production by using lignocellulosic waste from palm oil. The lignocellulosic waste used in this study is empty fruit branch of palm oil. It is pretreated with alkaline solution before undergoes fermentation process to produce cellulase. Pretreat...

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Main Author: Jong, Cheu Hann
Format: Undergraduate Final Project Report
Published: 2015
Online Access:http://discol.umk.edu.my/id/eprint/6117/
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spelling my.umk.eprints.61172022-05-23T08:45:15Z http://discol.umk.edu.my/id/eprint/6117/ Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste Jong, Cheu Hann This study is aim to determine production of cellulase production by using lignocellulosic waste from palm oil. The lignocellulosic waste used in this study is empty fruit branch of palm oil. It is pretreated with alkaline solution before undergoes fermentation process to produce cellulase. Pretreatment process able to break down the structure hard especially lignin to more simple substrate for Bacillus sp. consume. Bacillus subtilis IBRL A3 are selected as source of cellulase production bacteria, it give a better clear zone in congo red test. Extracted cellulase is filter by using filter paper to remove the substrate and centrifuge at 10000 rpm for 10 minutes to obtain the filtrate. Later filtrate is assay by using the DNS reagent and observes under spectrophotometer at 540 nm. There are three factor focused in production of cellulase, incubation time, temperature, inoculums size. Based on this study optimum of incubation time is at 72 hour which recorded as 7.76 U/g. While, optimum temperature to cellulase production is at 35°C recorded as 0.76 U/g. Besides that, optimum inoculums size found is 30% based on this study as recorded 9.72 U/g. Hence, production of cellulase optimum at 35°C, 30% inoculums size for 72 hour. 2015 Undergraduate Final Project Report NonPeerReviewed Jong, Cheu Hann (2015) Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This study is aim to determine production of cellulase production by using lignocellulosic waste from palm oil. The lignocellulosic waste used in this study is empty fruit branch of palm oil. It is pretreated with alkaline solution before undergoes fermentation process to produce cellulase. Pretreatment process able to break down the structure hard especially lignin to more simple substrate for Bacillus sp. consume. Bacillus subtilis IBRL A3 are selected as source of cellulase production bacteria, it give a better clear zone in congo red test. Extracted cellulase is filter by using filter paper to remove the substrate and centrifuge at 10000 rpm for 10 minutes to obtain the filtrate. Later filtrate is assay by using the DNS reagent and observes under spectrophotometer at 540 nm. There are three factor focused in production of cellulase, incubation time, temperature, inoculums size. Based on this study optimum of incubation time is at 72 hour which recorded as 7.76 U/g. While, optimum temperature to cellulase production is at 35°C recorded as 0.76 U/g. Besides that, optimum inoculums size found is 30% based on this study as recorded 9.72 U/g. Hence, production of cellulase optimum at 35°C, 30% inoculums size for 72 hour.
format Undergraduate Final Project Report
author Jong, Cheu Hann
spellingShingle Jong, Cheu Hann
Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
author_facet Jong, Cheu Hann
author_sort Jong, Cheu Hann
title Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
title_short Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
title_full Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
title_fullStr Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
title_full_unstemmed Production of cellulase by bacillus substilis through solid state fermentation using Lignocellulosic waste
title_sort production of cellulase by bacillus substilis through solid state fermentation using lignocellulosic waste
publishDate 2015
url http://discol.umk.edu.my/id/eprint/6117/
_version_ 1763303650705276928
score 13.211869

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