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Isolation and identification of novel bacteria producing cellulase

Cellulose is a large portion of the world's plant biomass. In nature, these polysaccharides are materials that require multiple enzymes to degrade them. The aim of this study was to find novel bacteria to degrade cellulose producing cellulose enzyme. Several samples were been isolated at soil a...

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Main Author: Muhamad Fateh Mustafa
Format: Undergraduate Final Project Report
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/5973/
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spelling my.umk.eprints.59732022-05-23T08:44:15Z http://discol.umk.edu.my/id/eprint/5973/ Isolation and identification of novel bacteria producing cellulase Muhamad Fateh Mustafa Cellulose is a large portion of the world's plant biomass. In nature, these polysaccharides are materials that require multiple enzymes to degrade them. The aim of this study was to find novel bacteria to degrade cellulose producing cellulose enzyme. Several samples were been isolated at soil and trunk of the dead wood. 3 samples from the decayed wood tree was shown a positive results. These bacteria sample B produce a large clearing zone indicated the high degradation rate which is 3.5cm followed by sample D which is 3.4cm and sample A and C which is 3.0cm. The cellulose assay then been futher analysis to prove the sample have a higher enzymatic activity. Sample B was chosen as the high enzymatic activity by producing 0.165 U/mI enzyme activity followed by sample A producing 0.124U/ml and sample D producing 0.111U/m1 enzyme activity. Bacillus cereus strain-JX847612, Bacillus cereus strain-KF641834 and Bacillus cereus strain-GU056811was identified by using gel electrophoresis method, DNA extraction method and sequenced using BLAST method. Thefore it is important to know the application of cellulose in industry in order to make useful of this research for beneficial. 2014 Undergraduate Final Project Report NonPeerReviewed Muhamad Fateh Mustafa (2014) Isolation and identification of novel bacteria producing cellulase. Undergraduate Final Project Report thesis, Faculty Of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Cellulose is a large portion of the world's plant biomass. In nature, these polysaccharides are materials that require multiple enzymes to degrade them. The aim of this study was to find novel bacteria to degrade cellulose producing cellulose enzyme. Several samples were been isolated at soil and trunk of the dead wood. 3 samples from the decayed wood tree was shown a positive results. These bacteria sample B produce a large clearing zone indicated the high degradation rate which is 3.5cm followed by sample D which is 3.4cm and sample A and C which is 3.0cm. The cellulose assay then been futher analysis to prove the sample have a higher enzymatic activity. Sample B was chosen as the high enzymatic activity by producing 0.165 U/mI enzyme activity followed by sample A producing 0.124U/ml and sample D producing 0.111U/m1 enzyme activity. Bacillus cereus strain-JX847612, Bacillus cereus strain-KF641834 and Bacillus cereus strain-GU056811was identified by using gel electrophoresis method, DNA extraction method and sequenced using BLAST method. Thefore it is important to know the application of cellulose in industry in order to make useful of this research for beneficial.
format Undergraduate Final Project Report
author Muhamad Fateh Mustafa
spellingShingle Muhamad Fateh Mustafa
Isolation and identification of novel bacteria producing cellulase
author_facet Muhamad Fateh Mustafa
author_sort Muhamad Fateh Mustafa
title Isolation and identification of novel bacteria producing cellulase
title_short Isolation and identification of novel bacteria producing cellulase
title_full Isolation and identification of novel bacteria producing cellulase
title_fullStr Isolation and identification of novel bacteria producing cellulase
title_full_unstemmed Isolation and identification of novel bacteria producing cellulase
title_sort isolation and identification of novel bacteria producing cellulase
publishDate 2014
url http://discol.umk.edu.my/id/eprint/5973/
_version_ 1763303630030503936
score 13.211869

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