Cover Image

Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave

Pitang leaves extract have been traditionally used as food colourant of purple pigment by Malay and some community in Kelantan. Thus, the study was conducted to determine the antioxidant potential and heavy metal content of pitang leaves extract. In order to obtain a purple pigment, pitang leaves we...

Full description

Saved in:
Bibliographic Details
Main Author: Mariah Al-Qibtiyah Omar
Format: Undergraduate Final Project Report
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/5958/
Tags: Add Tag
No Tags, Be the first to tag this record!
id my.umk.eprints.5958
record_format eprints
spelling my.umk.eprints.59582022-05-23T08:44:08Z http://discol.umk.edu.my/id/eprint/5958/ Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave Mariah Al-Qibtiyah Omar Pitang leaves extract have been traditionally used as food colourant of purple pigment by Malay and some community in Kelantan. Thus, the study was conducted to determine the antioxidant potential and heavy metal content of pitang leaves extract. In order to obtain a purple pigment, pitang leaves were extracted with methanol for three days and separated using liquid-liquid partition. Purple pigment was dried using rotoaveporator to obtain solid purple pigment. The antioxidant of solid purple pigment was determined by DPPH free radical scavenging assay. Then, the amount of heavy metal in the solid purple pigment was determined by Atomic Absorption Spectroscopy. Results indicated that the antioxidant activity of the pigment was IC₅₀ 930 μg/ml. The amount of heavy metals of Cu, Zn, Mg, K, Mn, Cd and Pb were 0.015 ± 0.007 ppm, 0.161 ± 0.002 ppm, 11.96 ± 0.029 ppm, 10.60 ± 0.483 ppm, 1.584 ± 0.010 ppm, 0.002 ± 0.007 ppm and 3.238 ± 0.020 ppm respectively. In conclusion, the purple pigment of pitang leaves have moderate antioxidant activity and the content of heavy metals were below the maximum permissible amount allowed by WHO; which indicate this pigment is safe to consume. 2014 Undergraduate Final Project Report NonPeerReviewed Mariah Al-Qibtiyah Omar (2014) Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Pitang leaves extract have been traditionally used as food colourant of purple pigment by Malay and some community in Kelantan. Thus, the study was conducted to determine the antioxidant potential and heavy metal content of pitang leaves extract. In order to obtain a purple pigment, pitang leaves were extracted with methanol for three days and separated using liquid-liquid partition. Purple pigment was dried using rotoaveporator to obtain solid purple pigment. The antioxidant of solid purple pigment was determined by DPPH free radical scavenging assay. Then, the amount of heavy metal in the solid purple pigment was determined by Atomic Absorption Spectroscopy. Results indicated that the antioxidant activity of the pigment was IC₅₀ 930 μg/ml. The amount of heavy metals of Cu, Zn, Mg, K, Mn, Cd and Pb were 0.015 ± 0.007 ppm, 0.161 ± 0.002 ppm, 11.96 ± 0.029 ppm, 10.60 ± 0.483 ppm, 1.584 ± 0.010 ppm, 0.002 ± 0.007 ppm and 3.238 ± 0.020 ppm respectively. In conclusion, the purple pigment of pitang leaves have moderate antioxidant activity and the content of heavy metals were below the maximum permissible amount allowed by WHO; which indicate this pigment is safe to consume.
format Undergraduate Final Project Report
author Mariah Al-Qibtiyah Omar
spellingShingle Mariah Al-Qibtiyah Omar
Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
author_facet Mariah Al-Qibtiyah Omar
author_sort Mariah Al-Qibtiyah Omar
title Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
title_short Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
title_full Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
title_fullStr Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
title_full_unstemmed Antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
title_sort antioxidant activity and heavy metals analysis in purple pigment extracted from gynochthodes sublanceolata leave
publishDate 2014
url http://discol.umk.edu.my/id/eprint/5958/
_version_ 1763303627910283264
score 13.211869

Similar Items