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Screening and identification of bacteria producing lignin degrading enzymes

Lignin is the most abundant renewable biopolymer material on earth. Millions of tons of lignin and lignin-related compounds are produced as waste effluent from the pulping and paper industries. Physico-chemical methods used to treat lignin wastewater result from the paper mill manufacturing were not...

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Main Author: Iylia Abd Halim
Format: Undergraduate Final Project Report
Published: 2014
Online Access:http://discol.umk.edu.my/id/eprint/5953/
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spelling my.umk.eprints.59532022-05-23T08:44:07Z http://discol.umk.edu.my/id/eprint/5953/ Screening and identification of bacteria producing lignin degrading enzymes Iylia Abd Halim Lignin is the most abundant renewable biopolymer material on earth. Millions of tons of lignin and lignin-related compounds are produced as waste effluent from the pulping and paper industries. Physico-chemical methods used to treat lignin wastewater result from the paper mill manufacturing were not practically effective and expensive. Biological treatment was found to be the alternative method using bacteria as a lignin degrader. The aim of this project is to screen and identify potential microorganisms producing lignin-degrading enzyme as well as the enzyme activity assay. Four strain of bacteria were chosen as they were able to grow on selective agar medium containing lignin as a carbon source and clear zone formation. The four strain of bacteria was then identified as a Bacillus cereus (GU551935), Bacillus sp. (JX847615). Berta Imbellis (AF519651) and Bacillus cereus (JX84612). These bacteria were subjected to cultivate in a basal medium to analyse the lignolytic enzyme produced. Bacillus sp. (JX847615) were found to produce a higher amount of enzyme activity of LiP (1.383 x 10‐⁵U/m1) followed by MnP (9.17 x 10‐⁷ Wm) when grown in basal medium. 2014 Undergraduate Final Project Report NonPeerReviewed Iylia Abd Halim (2014) Screening and identification of bacteria producing lignin degrading enzymes. Undergraduate Final Project Report thesis, Faculty Of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Lignin is the most abundant renewable biopolymer material on earth. Millions of tons of lignin and lignin-related compounds are produced as waste effluent from the pulping and paper industries. Physico-chemical methods used to treat lignin wastewater result from the paper mill manufacturing were not practically effective and expensive. Biological treatment was found to be the alternative method using bacteria as a lignin degrader. The aim of this project is to screen and identify potential microorganisms producing lignin-degrading enzyme as well as the enzyme activity assay. Four strain of bacteria were chosen as they were able to grow on selective agar medium containing lignin as a carbon source and clear zone formation. The four strain of bacteria was then identified as a Bacillus cereus (GU551935), Bacillus sp. (JX847615). Berta Imbellis (AF519651) and Bacillus cereus (JX84612). These bacteria were subjected to cultivate in a basal medium to analyse the lignolytic enzyme produced. Bacillus sp. (JX847615) were found to produce a higher amount of enzyme activity of LiP (1.383 x 10‐⁵U/m1) followed by MnP (9.17 x 10‐⁷ Wm) when grown in basal medium.
format Undergraduate Final Project Report
author Iylia Abd Halim
spellingShingle Iylia Abd Halim
Screening and identification of bacteria producing lignin degrading enzymes
author_facet Iylia Abd Halim
author_sort Iylia Abd Halim
title Screening and identification of bacteria producing lignin degrading enzymes
title_short Screening and identification of bacteria producing lignin degrading enzymes
title_full Screening and identification of bacteria producing lignin degrading enzymes
title_fullStr Screening and identification of bacteria producing lignin degrading enzymes
title_full_unstemmed Screening and identification of bacteria producing lignin degrading enzymes
title_sort screening and identification of bacteria producing lignin degrading enzymes
publishDate 2014
url http://discol.umk.edu.my/id/eprint/5953/
_version_ 1763303627193057280
score 13.211869

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