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Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor

In this study, three physical parameters were selected to study its effect on the production of thermostable 50a protease and were then identified the optimal conditions of physical parameters. Selected parameters were incubation time, agitation rate and inoculum size. The effect of physical paramet...

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Main Author: Nuramirah Azali
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5786/
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spelling my.umk.eprints.57862022-05-23T08:43:09Z http://discol.umk.edu.my/id/eprint/5786/ Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor Nuramirah Azali In this study, three physical parameters were selected to study its effect on the production of thermostable 50a protease and were then identified the optimal conditions of physical parameters. Selected parameters were incubation time, agitation rate and inoculum size. The effect of physical parameters to Thermostable 50a protease production was optimized by using the conventional method of one factor at a time (OFAT). Cultivation is done in a volume of 2 L bioreactor at constant conditions: temperature 37 ° C and pH 7 during the incubation. The first physical parameter studied was incubation time, followed by agitation rate and inoculum size. The ranges used were: incubation time (0 hours to 56 hours), agitation rate (0 rpm to 200 rpm) and inoculum size (2.5% to 10%). The optimized content of thermostable 50a protease was 233.222 Um1ˉ¹ and bacterial growth of 1.346 at optical density (OD) 450 nm were obtained with optimal parameters conditions: incubation time (46 hours), agitation rate (150 rpm) and inoculum size (2.5%). These results showed that, by knowing the optimal physical parameters condition, it will give advantages to produce high quantities of thermostable 50a protease and can reduce time consuming and also give it an edge on cost reduction, particularly in the industrial scale. 2013 Undergraduate Final Project Report NonPeerReviewed Nuramirah Azali (2013) Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description In this study, three physical parameters were selected to study its effect on the production of thermostable 50a protease and were then identified the optimal conditions of physical parameters. Selected parameters were incubation time, agitation rate and inoculum size. The effect of physical parameters to Thermostable 50a protease production was optimized by using the conventional method of one factor at a time (OFAT). Cultivation is done in a volume of 2 L bioreactor at constant conditions: temperature 37 ° C and pH 7 during the incubation. The first physical parameter studied was incubation time, followed by agitation rate and inoculum size. The ranges used were: incubation time (0 hours to 56 hours), agitation rate (0 rpm to 200 rpm) and inoculum size (2.5% to 10%). The optimized content of thermostable 50a protease was 233.222 Um1ˉ¹ and bacterial growth of 1.346 at optical density (OD) 450 nm were obtained with optimal parameters conditions: incubation time (46 hours), agitation rate (150 rpm) and inoculum size (2.5%). These results showed that, by knowing the optimal physical parameters condition, it will give advantages to produce high quantities of thermostable 50a protease and can reduce time consuming and also give it an edge on cost reduction, particularly in the industrial scale.
format Undergraduate Final Project Report
author Nuramirah Azali
spellingShingle Nuramirah Azali
Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
author_facet Nuramirah Azali
author_sort Nuramirah Azali
title Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
title_short Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
title_full Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
title_fullStr Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
title_full_unstemmed Study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
title_sort study of physical parameters for efficient production of thermostable 50a protease using 2 l bioreactor
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5786/
_version_ 1763303603026526208
score 13.211869

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