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Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.

The present study is to determine the antibacterial activity and antifouling proper-ties from carapace of female mud crab, Scylla serrata. This is a preliminary study as there are no literature reviews or researches available in screening of bioactive compound from female mud crab, Scylla serrata. T...

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Main Author: Nishalline Manoharan
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5772/
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spelling my.umk.eprints.57722022-05-23T08:43:05Z http://discol.umk.edu.my/id/eprint/5772/ Screening of Crude extracts compound from carapace of female mud crab, scylla serrata. Nishalline Manoharan The present study is to determine the antibacterial activity and antifouling proper-ties from carapace of female mud crab, Scylla serrata. This is a preliminary study as there are no literature reviews or researches available in screening of bioactive compound from female mud crab, Scylla serrata. Therefore this study was carried out in order to determine whether female carapace of mud crab, Scylla serrata contains bioactive compounds which have the ability to deteriorate the pharmaceutical industry, bacterial strains tendency to generate resistance and also whether it would pose health hazard to the consumers. Three different extraction solvent were used namely; methanol, acetone and ethyl acetate to extract the bioactive compound from female carapace. Four species of bacteria of Gram-positive and Gram-negative were used which are Salmonella typhimurium, Escherichia coli, Staphylococcus aureus and Bacillus cereus. The antibacterial activity was determined by using Disc Diffusion Test (DDT) method. Out of three solvent, only methanol crude extraction shows zone of inhibition among four species of bacteria. Meanwhile, antifouling test was carried out to detect attachment and growth of bacteria on surface immersed in seawater. Staining process was took place to determine the clear glass slides. Unfortunately, there were no clear glass slides found among three crude extracted solvent in four species of bacteria. However, further research is required to justify these preliminary findings. The results obtained can be used as a guideline for further research in this field at the future. 2013 Undergraduate Final Project Report NonPeerReviewed Nishalline Manoharan (2013) Screening of Crude extracts compound from carapace of female mud crab, scylla serrata. Undergraduate Final Project Report thesis, Faculty of Agro Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description The present study is to determine the antibacterial activity and antifouling proper-ties from carapace of female mud crab, Scylla serrata. This is a preliminary study as there are no literature reviews or researches available in screening of bioactive compound from female mud crab, Scylla serrata. Therefore this study was carried out in order to determine whether female carapace of mud crab, Scylla serrata contains bioactive compounds which have the ability to deteriorate the pharmaceutical industry, bacterial strains tendency to generate resistance and also whether it would pose health hazard to the consumers. Three different extraction solvent were used namely; methanol, acetone and ethyl acetate to extract the bioactive compound from female carapace. Four species of bacteria of Gram-positive and Gram-negative were used which are Salmonella typhimurium, Escherichia coli, Staphylococcus aureus and Bacillus cereus. The antibacterial activity was determined by using Disc Diffusion Test (DDT) method. Out of three solvent, only methanol crude extraction shows zone of inhibition among four species of bacteria. Meanwhile, antifouling test was carried out to detect attachment and growth of bacteria on surface immersed in seawater. Staining process was took place to determine the clear glass slides. Unfortunately, there were no clear glass slides found among three crude extracted solvent in four species of bacteria. However, further research is required to justify these preliminary findings. The results obtained can be used as a guideline for further research in this field at the future.
format Undergraduate Final Project Report
author Nishalline Manoharan
spellingShingle Nishalline Manoharan
Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
author_facet Nishalline Manoharan
author_sort Nishalline Manoharan
title Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
title_short Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
title_full Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
title_fullStr Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
title_full_unstemmed Screening of Crude extracts compound from carapace of female mud crab, scylla serrata.
title_sort screening of crude extracts compound from carapace of female mud crab, scylla serrata.
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5772/
_version_ 1763303600952442880
score 13.211869

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