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Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)

Protein and protease from three selected germinated seedlings; Maize (Zea mays L.), Mung Beans (Vigna radiata L.) and Red Kidney Bean (Phaseolus vulgaris L) was purified. The methods were partially purification using Ammonium Sulphate Precipitation and Dialysis. The protein and protease were purifie...

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Main Author: Ali Mohamad Yasin
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5764/
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spelling my.umk.eprints.57642022-05-23T08:43:03Z http://discol.umk.edu.my/id/eprint/5764/ Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.) Ali Mohamad Yasin Protein and protease from three selected germinated seedlings; Maize (Zea mays L.), Mung Beans (Vigna radiata L.) and Red Kidney Bean (Phaseolus vulgaris L) was purified. The methods were partially purification using Ammonium Sulphate Precipitation and Dialysis. The protein and protease were purified using Ion Exchange Chromatography where DEAF-Cellulose acted as the resin and Size Exclusion Chromatography with Sephadex-G100 resin was used. The study was used Bradford's Protein Assay in the determination of the protein concentration after series of purification. Azocasein was used as substrate in assaying the activity of the protease. The study found Red Kidney Bean showed the highest protease activity with 34.667 thrill respectively followed by Maize and Mung Bean. Besides that, it also had the highest protein concentration compared to others after the purification using Size Exclusion Chromatography. The SDS PAGE analysis revealed that the molecular weight of Red Kidney Bean's Protein was 36 kDa, 48 kDa for Mung Bean and the estimation value for the Maize's protein sample was 40 kDa This result showed that Phaseolus vulgaris L. under study is a good producer of extra cellular protease, which can be beneficial for enzymatic industries and the consumers. 2013 Undergraduate Final Project Report NonPeerReviewed Ali Mohamad Yasin (2013) Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.). Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description Protein and protease from three selected germinated seedlings; Maize (Zea mays L.), Mung Beans (Vigna radiata L.) and Red Kidney Bean (Phaseolus vulgaris L) was purified. The methods were partially purification using Ammonium Sulphate Precipitation and Dialysis. The protein and protease were purified using Ion Exchange Chromatography where DEAF-Cellulose acted as the resin and Size Exclusion Chromatography with Sephadex-G100 resin was used. The study was used Bradford's Protein Assay in the determination of the protein concentration after series of purification. Azocasein was used as substrate in assaying the activity of the protease. The study found Red Kidney Bean showed the highest protease activity with 34.667 thrill respectively followed by Maize and Mung Bean. Besides that, it also had the highest protein concentration compared to others after the purification using Size Exclusion Chromatography. The SDS PAGE analysis revealed that the molecular weight of Red Kidney Bean's Protein was 36 kDa, 48 kDa for Mung Bean and the estimation value for the Maize's protein sample was 40 kDa This result showed that Phaseolus vulgaris L. under study is a good producer of extra cellular protease, which can be beneficial for enzymatic industries and the consumers.
format Undergraduate Final Project Report
author Ali Mohamad Yasin
spellingShingle Ali Mohamad Yasin
Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
author_facet Ali Mohamad Yasin
author_sort Ali Mohamad Yasin
title Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
title_short Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
title_full Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
title_fullStr Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
title_full_unstemmed Biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
title_sort biochemical analysis of leguminous seedings of maize ( zea mays l.), mung bean (vigna radiata l.) and red kidney bean (phaseolus vulgaris l.)
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5764/
_version_ 1763303599784329216
score 13.211869

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