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Study on the sterilization method and micropropagation of rubber tree

This study was conducted to determine the best sterilization method for Hevea brasiliensis and the concentration of hormonal regulation of plant combinations are best suited for the development and growth of rubber trees through tissue culture methods. The major problem faced in the study is bacteri...

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Main Author: Kasyifah Ibrahim
Format: Undergraduate Final Project Report
Published: 2013
Online Access:http://discol.umk.edu.my/id/eprint/5660/
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spelling my.umk.eprints.56602022-05-23T08:42:33Z http://discol.umk.edu.my/id/eprint/5660/ Study on the sterilization method and micropropagation of rubber tree Kasyifah Ibrahim This study was conducted to determine the best sterilization method for Hevea brasiliensis and the concentration of hormonal regulation of plant combinations are best suited for the development and growth of rubber trees through tissue culture methods. The major problem faced in the study is bacterial contamination. It might due to contaminated explant used with bacteria and other microorganisms. Different sterilization methods were tested to overcome this problem. Based on this parameter, the best result was obtained with the combination of 80% (v/v) of ethanol for 20 seconds and 4% (v/v) NaOCI for 3 minutes (Method 2). Further study were carried out to investigate the effect of cytokinin on shoot initiation in three different concentration of BAP/Kn/2ip and constant NAA/IAA/2 BAP/Kn/2ip,4-D. Result shown that MS media supplemented with 0.5mg/L IAA + 1.0mg / 1 2ip give shoot after 2 weeks of culture. Further studies on Hevea brasiliensis should be carried out to determine the best conditions for shoots regeneration in Hevea brasiliensis. 2013 Undergraduate Final Project Report NonPeerReviewed Kasyifah Ibrahim (2013) Study on the sterilization method and micropropagation of rubber tree. Undergraduate Final Project Report thesis, Faculty of Agro - Based Industry. (Submitted)
institution Universiti Malaysia Kelantan
building Perpustakaan Universiti Malaysia Kelantan
collection Institutional Repository
continent Asia
country Malaysia
content_provider Universiti Malaysia Kelantan
content_source UMK Institutional Repository
url_provider http://umkeprints.umk.edu.my/
description This study was conducted to determine the best sterilization method for Hevea brasiliensis and the concentration of hormonal regulation of plant combinations are best suited for the development and growth of rubber trees through tissue culture methods. The major problem faced in the study is bacterial contamination. It might due to contaminated explant used with bacteria and other microorganisms. Different sterilization methods were tested to overcome this problem. Based on this parameter, the best result was obtained with the combination of 80% (v/v) of ethanol for 20 seconds and 4% (v/v) NaOCI for 3 minutes (Method 2). Further study were carried out to investigate the effect of cytokinin on shoot initiation in three different concentration of BAP/Kn/2ip and constant NAA/IAA/2 BAP/Kn/2ip,4-D. Result shown that MS media supplemented with 0.5mg/L IAA + 1.0mg / 1 2ip give shoot after 2 weeks of culture. Further studies on Hevea brasiliensis should be carried out to determine the best conditions for shoots regeneration in Hevea brasiliensis.
format Undergraduate Final Project Report
author Kasyifah Ibrahim
spellingShingle Kasyifah Ibrahim
Study on the sterilization method and micropropagation of rubber tree
author_facet Kasyifah Ibrahim
author_sort Kasyifah Ibrahim
title Study on the sterilization method and micropropagation of rubber tree
title_short Study on the sterilization method and micropropagation of rubber tree
title_full Study on the sterilization method and micropropagation of rubber tree
title_fullStr Study on the sterilization method and micropropagation of rubber tree
title_full_unstemmed Study on the sterilization method and micropropagation of rubber tree
title_sort study on the sterilization method and micropropagation of rubber tree
publishDate 2013
url http://discol.umk.edu.my/id/eprint/5660/
_version_ 1763303584762429440
score 13.211869

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